Abstract
Eighteen polymorphic microsatellite loci were isolated from the vulnerable Chinese egret Egretta eulophotes using the magnetic bead-based enrichment method, and were tested in a sample of 20 individuals from a Chinese egret population distributed in Fujian province of China. The number of alleles range from 2 to 9 per locus with the observed and expected heterozygosity ranging from 0.20 to 0.85 and 0.18 to 0.82, respectively. One locus deviated significantly from Hardy–Weinberg equilibrium and no pairs showed evidence of linkage disequilibrium after sequential bonferroni correction. These loci were also confirmed useful for the cross-amplifications in other five ardeid species. These new microsatellite markers will be useful for the further studies on the genetic structure, molecular evolution and conservation management of this vulnerable species and the other ardeid species.
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Acknowledgments
This research was funded by the National Natural Science Foundation of China (Grant code 30570254 and 40876077), by the Fujian Natural Science Foundation of China (Grant code 2007J0140 and 2008S0007) and by the Program for Innovative Research Team in Science and Technology in Fujian Province University.
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Huang, X., Zhou, X., Chen, M. et al. Isolation and characterization of microsatellite loci in vulnerable Chinese egret (Egretta eulophotes: Aves). Conserv Genet 11, 1211–1214 (2010). https://doi.org/10.1007/s10592-009-9921-1
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DOI: https://doi.org/10.1007/s10592-009-9921-1