Abstract
Primula sieblodii E. Morren is a perennial herb, classified as ‘Near Threatened’ in the Japanese Red List. Using the magnetic enrichment method, we have developed 120 microsatellite markers and screened polymorphisms within the species as a whole as well as within a single population. At the species level, the average number of alleles per locus, and the average observed and expected heterozygosities were 7.06, 0.48, and 0.74, respectively; at the level of the single population these values were 5.53, 0.52, and 0.64, respectively. The microsatellite markers presented here will be valuable research tools in forthcoming projects, such as QTL mapping as well as for association studies of P. sieboldii.
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Acknowledgments
We thank Mihoko Nagai for P. sieboldii samples and Kensuke Yoshimura for server management. Financial support was provided by a grant from JSPS Research Fellowships for Young Scientists to S. U. (03J08415), the Environmental Research and Technology Development Fund from the Ministry of the Environment, Government of Japan and KAKENHI (20241055).
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Ueno, S., Yoshida, Y., Taguchi, Y. et al. Development of 120 microsatellite markers for Primula sieboldii E. Morren for linkage mapping. Conserv Genet 10, 1945 (2009). https://doi.org/10.1007/s10592-009-9861-9
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DOI: https://doi.org/10.1007/s10592-009-9861-9