Abstract
Piper polysyphonum is an endemic species in southeast Asia, in the narrow habitat located in the Chinese provinces of Guizhou and Yunnan, and the country of Laos. Recently, loss of forests due to agricultural activity has dramatically reduced the habitat and population size of P. polysyphonum. In this study, eleven primer sets of polymorphic microsatellite DNA loci were developed for P. polysyphonum. Allele numbers ranged from two to ten, with observed heterozygosities ranging from 0.222 to 0.889. Four loci exhibited a departure from Hardy–Weinberg equilibrium, possibly due to population admixture. No loci pairs revealed significant linkage disequilibrium. Among the eleven loci, two with extremely high numbers of TCG repeats were obtained. The polymorphic microsatellite DNA markers reported here should provide a helpful means to address questions concerning population structure and demographic history of P. polysyphonum for conservation efforts.
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References
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50
Hearne CM, Ghosh S, Todd JA (1992) Microsatellites for linkage analysis of genetic traits. Trends Genet 8:288–294
Jaramillo MA, Callejas R (2004) Chapter 10. Current perspectives on the classification and phylogenetics of the genus Piper L. In: Dyer LA, Palmer DN (eds) Piper: a model genus for studies of phytochemistry, ecology, and evolution. Kluwer Academic/Plenum Publishers, New York, pp 179–198
Jaramillo MA, Manos PS (2001) Phylogeny and patterns of floral diversity in the genus Piper (Piperaceae). Am J Bot 88:706–716. doi:10.2307/2657072
Ma Y, Han GQ (1993) Cyclohexene epoxides from Piper polysyphorum. J Chin Pharm Sci 2:97–101
Ma Y, Han GQ, Li CL, Cheng JR, Arison BH, Hwang SB (1991) Neolignans from Piper polysyphorum C. DC. Acta Pharmacol Sin 26:345–350
Pannell JR, Dorken ME (2006) Colonisation as a common denominator in plant metapopulations and range expansions: effects on genetic diversity and sexual systems. Landsc Ecol 21:837–848. doi:10.1007/s10980-005-5389-7
Prabhu MS, Platel K, Saraswathi G, Srinivasan K (1995) Effect of orally administered betel leaf (Piper betle Linn.) on digestive enzymes of pancreas and intestinal mucosa and on bile production in rats. Indian J Exp Biol 33:752–756
Quijano-Abril MA, Callejas-Posada R, Miranda-Esquivel DR (2006) Areas of endemism and distribution patterns for Neotropical Piper species (Piperaceae). J Biogeogr 33:1266–1278. doi:10.1111/j.1365-2699.2006.01501.x
Raymond M, Rousset F (1995) Genepop: population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Shu HJ (1999) Piper. In: Flora of China 4. Missouri Botanical Garden, St. Louis, MO & Harvard University Herbaria, Cambridge, MA, pp 110–129
Sork VL, Smouse PE (2006) Genetic analysis of landscape connectivity in tree populations. Landsc Ecol 21:821–836. doi:10.1007/s10980-005-5415-9
Wanke S, Jaramillo MA, Borsch T, Samain MS, Quandt D, Neinhuis C (2007) Evolution of Piperales—matK gene and trnK intron sequence data reveal lineage specific resolution contrast. Mol Phylogenet Evol 42:477–497. doi:10.1016/j.ympev.2006.07.007
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16. doi:10.1046/j.0962-1083.2001.01418.x
Zhang K, Chen C, Chen D (1997) Chemical constituents of Piper polysyphorum. Acta Bot Yunnanica 19:211–212
Acknowledgments
This study was supported by grants from the National Science Council of the ROC on Taiwan awarded to Yu-Chung Chiang.
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Pei-Chun Liao, Xun Gong and Huei-Chuan Shih contributed equally to this work.
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Liao, PC., Gong, X., Shih, HC. et al. Isolation and characterization of eleven polymorphic microsatellite loci from an endemic species, Piper polysyphonum (Piperaceae). Conserv Genet 10, 1911–1914 (2009). https://doi.org/10.1007/s10592-009-9852-x
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DOI: https://doi.org/10.1007/s10592-009-9852-x