Abstract
The first set of nuclear simple sequence repeat (SSR) loci for Brachiaria brizantha (Hochst. ex A. Rich.) Stap is described. A microsatellite-enriched library was constructed and 19 loci were characterized. About 13 SSR loci were found to be polymorphic and across-taxa amplification tests showed that six of them can be transferred to four other species of Brachiaria. This new SSR resource will be a powerful tool for population genetic studies of B. brizantha, for interspecific genetic studies within the genus Brachiaria, for mapping and for marker assisted selection in breeding.
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Acknowledgments
Authors would like to thank Renato Vicentini dos Santos for bioinformatics support for the project. This work was realized with grants from Brazilian Agricultural Research Corporation (Embrapa), Brazilian National Council for Scientific and Technological Development (CNPq), Research Foundation of the State of São Paulo (Fapesp) and Foundation for Science and Technological Development of the State of Mato Grosso do Sul (Fundect).
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Jungmann, L., Sousa, A.C.B., Paiva, J. et al. Isolation and characterization of microsatellite markers for Brachiaria brizantha (Hochst. ex A. Rich.) Stap. Conserv Genet 10, 1873 (2009). https://doi.org/10.1007/s10592-009-9839-7
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DOI: https://doi.org/10.1007/s10592-009-9839-7