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Isolation and characterization of twenty novel microsatellite markers for half-smooth tongue sole (Cynoglossus semilaevis)

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Abstract

Twenty novel microsatellite markers for half-smooth tongue sole (Cynoglossus semilaevis) were isolated and characterized from a simple sequence repeat-enriched library constructed with (GA)15 and (CA)15. The polymorphism was assessed using 32 individuals collected from one locality along the Chinese coast. The result showed the number of alleles ranged from 2 to 14, with an average of 8.7 alleles/locus. The values of observed and expected heterozygosities ranged from 0.2609 to 1.0000 and from 0.2367 to 0.9716, respectively. Six microsatellite loci departed significantly from Hardy–Weinberg equilibrium. No linkage disequilibrium was found. These markers will be useful for the conservation studies and population structure assessment for this species.

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Acknowledgments

We would like to thank Dr. AiBin Zhan (The Ohio State University, USA) for technical assistance and commenting on the early version of this manuscript. This work was also supported by grants from the National High Technology Research and Development Program of China (No. 2006AA10A404, 2006AA10A414) and the National Natural Science Foundation of China (No. 30671624, 30600455).

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Authors and Affiliations

  1. Laboratory of Marine Genetics and Breeding, College of Marine Life Science, Ocean University of China, No.5 Yushan Road, 266003, Qingdao, People’s Republic of China

    Qiwang Zhong, Yan Yu, Quanqi Zhang, Zhigang Wang, Haiyang Yu & Jiashi Wang

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  1. Qiwang Zhong
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  2. Yan Yu
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  3. Quanqi Zhang
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  4. Zhigang Wang
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  5. Haiyang Yu
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  6. Jiashi Wang
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Corresponding author

Correspondence to Quanqi Zhang.

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Q. Zhong and Y. Yu contributed equally to this work.

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Zhong, Q., Yu, Y., Zhang, Q. et al. Isolation and characterization of twenty novel microsatellite markers for half-smooth tongue sole (Cynoglossus semilaevis). Conserv Genet 10, 1617–1620 (2009). https://doi.org/10.1007/s10592-009-9809-0

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  • DOI: https://doi.org/10.1007/s10592-009-9809-0

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