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Development and characterization of microsatellite markers for Chinese bayberry (Myrica rubra Sieb. & Zucc.)

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Abstract

Chinese bayberry (Myrica rubra Sieb. & Zucc.) is one of the most important fruit tree crops in southern China. This paper reports the development of microsatellite primers for Chinese bayberry. By screening the expressed sequence tag database and other sources, 11 polymorphic loci were generated and primers were designed. Polymorphism of these 11 loci was assessed in 32 cultivars. All the 11 loci are polymorphic and the number of alleles (A) ranged from 3 to 12, observed heterozygosity (H O) and expected heterozygosity (H E) ranged from 0.1250 to 0.9667 and 0.2359 to 0.8790, respectively, polymorphism information content ranged from 0.2285 to 0.8516. These microsatellite loci should be useful in the studies of genetic diversity of Myrica rubra and will provide useful implications for resource conservation.

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Acknowledgments

This work was supported by the Science and Technology Project of Zhejiang Province (2006C14016), the Ningbo Science and Technology Project (2005C100108), and the 111 project (B06014).

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Authors and Affiliations

  1. Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Huajiachi Campus, 310029, Hangzhou, People’s Republic of China

    Shui Ming Zhang, Chang Jie Xu, Zhong Shan Gao & Kun Song Chen

  2. Department of Horticulture, Anhui Agricultural University, 230036, Hefei, People’s Republic of China

    Shui Ming Zhang

  3. Yuyao Agricultural and Forestry Bureau, 315400, Yuyao, People’s Republic of China

    Guo Yun Wang

Authors
  1. Shui Ming Zhang
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  2. Chang Jie Xu
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  3. Zhong Shan Gao
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  4. Kun Song Chen
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  5. Guo Yun Wang
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Corresponding author

Correspondence to Zhong Shan Gao.

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Zhang, S.M., Xu, C.J., Gao, Z.S. et al. Development and characterization of microsatellite markers for Chinese bayberry (Myrica rubra Sieb. & Zucc.). Conserv Genet 10, 1605–1607 (2009). https://doi.org/10.1007/s10592-008-9804-x

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  • DOI: https://doi.org/10.1007/s10592-008-9804-x

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