Abstract
The present study reports isolation and characterization of 21 polymorphic microsatellite markers developed from Lycium chinense Mill. These markers produced a total of 86 alleles across 30 L. chinense accessions, with an average of 4.1 alleles per locus. Values for observed heterozygosity and polymorphism information content ranged from 0.03 to 0.81 (mean = 0.35) and from 0.03 to 0.78 (mean = 0.31), respectively. At the significance threshold (P < 0.05), 12 loci deviated from Hardy–Weinberg equilibrium, whereas significant linkage disequilibrium values were observed between 52 pairs of loci. All loci were successfully amplified for all L. barbarum accessions. These newly developed polymorphic microsatellite markers will be very useful for programming in the genetic conservation and classification of L. chinense and L. barbarum.
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Acknowledgments
This study was supported by Biogreen 21 project (#20080401034058) of the Rural Development Administration (RDA) and a grant (Code #200803101010290) from National Institute of Agricultural Biotechnology, RDA, Republic of Korea.
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Kwon, SJ., Lee, GA., Lee, SY. et al. Isolation and characterization of 21 microsatellite loci in Lycium chinense and cross-amplification in Lycium barbarum . Conserv Genet 10, 1557 (2009). https://doi.org/10.1007/s10592-008-9792-x
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DOI: https://doi.org/10.1007/s10592-008-9792-x