Abstract
Fagus crenata Blume is widely distributed throughout Japanese cool-temperate deciduous broad-leaved forests, but there are two divergent groups of populations in areas with contrasting winter climates separated by Japan’s Central Mountain Range. To facilitate investigations of adaptive genetic differentiation of the species using potentially functional genes, we have collected Expressed Sequence Tags and developed Simple Sequence Repeat markers using a cDNA library constructed from cambium and surrounding tissues. In total, 270 primer pairs were designed, and 87 of the corresponding loci showed polymorphism in 16 individuals, with 2–21 alleles per locus and expected heterozygosities ranging from 0.06 to 0.97. EST-SSR markers developed in the present study will be useful for genomic analyses of F. crenata populations.
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Acknowledgments
The authors are grateful to Ms. Koshiba and Mr. Komatsu for laboratory work. This research was supported by KAKENHI (20380089) and a grant for Research on Genetic Guidelines for Restoration Programs using Genetic Diversity Information from the Ministry of Environment, Japan.
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Ueno, S., Taguchi, Y., Tomaru, N. et al. Development of EST-SSR markers from an inner bark cDNA library of Fagus crenata (Fagaceae) . Conserv Genet 10, 1477 (2009). https://doi.org/10.1007/s10592-008-9764-1
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DOI: https://doi.org/10.1007/s10592-008-9764-1