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Isolation and characterization of fifteen polymorphic microsatellite loci from Primula merrilliana (Primulaceae), an endemic from China

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Abstract

Primula merrilliana (Primulaceae) is an endangered plant endemic to south Anhui Province in China. In this study, we isolated fifteen polymorphic microsatellite loci from a population of 22 individuals using a modified biotin-capture method. Our isolated loci provided microsatellite markers with polymorphism of 3–11 alleles per locus. The expected and observed heterozygosities ranged from 0.392 to 0.855 and from 0.136 to 1.000, respectively. These markers would be the useful tools for analyzing questions concerning population genetic structure and mating system of Primula merrilliana.

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Acknowledgments

This research was financially supported by the National Natural Science Foundation of China (No.30500300; 30870062), the New Century Excellent Talents in University of the Education Ministry of China (NCET-06-0558), the Key Project of Chinese Ministry of Education (NO.206066), the Anhui Excellent Youth Foundation of Science and Technology (No. 06043089), the Recruiting Overseas Talents Foundation of Anhui Province (No.2005Z032) and the Key Grant of Anhui Bureau of Education (NO.2006KJ061A).

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Authors and Affiliations

  1. College of Life Science, the Key Laboratory of Molecular Evolution and Biodiversity and the Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Anhui Normal University, No. 1 Eastern Beijing Road, 241000, Wuhu, People’s Republic of China

    Yan-qiu Peng, Jian-wen Shao, Hai-long Wu, Xiao-ping Zhang & Guo-ping Zhu

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  1. Yan-qiu Peng
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  2. Jian-wen Shao
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  3. Hai-long Wu
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  4. Xiao-ping Zhang
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  5. Guo-ping Zhu
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Correspondence to Guo-ping Zhu.

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Peng, Yq., Shao, Jw., Wu, Hl. et al. Isolation and characterization of fifteen polymorphic microsatellite loci from Primula merrilliana (Primulaceae), an endemic from China. Conserv Genet 10, 1441–1443 (2009). https://doi.org/10.1007/s10592-008-9756-1

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  • DOI: https://doi.org/10.1007/s10592-008-9756-1

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