Abstract
Primulina tabacum is a rare and endangered perennial herb with highly restricted limestone distribution in southern China. To enrich our scientific conservation for this species, we developed ten microsatellite markers using repetitive DNA enriched libraries. The number of alleles per microsatellite locus varied from two to six. The expected (H E) and observed (H O) heterozygosities varied from 0.4059 to 0.7294 and from 0.1364 to 0.5217, respectively. These markers will be employed in future studies of genetic structure in P. tabacum.
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Acknowledgments
We thank Shi-Jin Li for helping collect Primulina tabacum samples. This work was supported by National Basic Research Program of China (973 Program) (No. 2007CB411600), Guangdong Key Technologies R&D Program—Sci-Tech Basic Platform project (2007A060306011), Guangdong & Guangzhou Sci-Tech Planning Project (2006B60101034, 07118249, 2007J1-C0471)
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Zheng-Feng Wang and Hai Ren are joint first author
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Wang, ZF., Ren, H., Zhang, QM. et al. Isolation and characterization of microsatellite markers for Primulina tabacum, a critically endangered perennial herb. Conserv Genet 10, 1433–1435 (2009). https://doi.org/10.1007/s10592-008-9754-3
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DOI: https://doi.org/10.1007/s10592-008-9754-3