Skip to main content
Log in

Isolation and characterization of polymorphic microsatellite markers for the brown-headed nuthatch (Sitta pusilla)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Populations of brown-headed nuthatches (Sitta pusilla) are declining throughout the species range. Here we characterize twelve polymorphic microsatellite loci for this species. Analysis of 32 presumably unrelated individuals from a single population revealed an average of 14.9 alleles per locus (range 4–25), an average observed heterozygosity of 0.74 (range 0.52–0.94) and an average polymorphic information content of 0.80 (range 0.57–0.95). We anticipate that these microsatellite markers will be useful for population genetic and behavioral studies on the brown-headed nuthatch and closely related species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Brazeau D, Clark AM (2005) Molecular markers: tools for developing enriched microsatellite libraries. ICBR, University of Florida, Gainesville, FL

    Google Scholar 

  • Brownstein MJ, Carpenter JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq polymerase: primer modifications that facilitate genotyping. Biotechniques 20:1004–1010

    CAS  PubMed  Google Scholar 

  • Kalinowski WT, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106. doi:10.1111/j.1365-294X.2007.03089.x

    Article  PubMed  Google Scholar 

  • Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers. Proc Natl Acad Sci USA 91:88–92. doi:10.1073/pnas.91.1.88

    Article  CAS  PubMed  Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225. doi:10.2307/2409177

    Article  Google Scholar 

  • Rousset F (2008) GENEPOP '007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106. doi:10.1111/j.1471-8286.2007.01931.x

    Article  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

  • Walters J, Cooper C, Daniels S, Pasinelli G, Schiegg K (2004) Conservation Biology. In: Koenig W, Dickinson J (eds) Ecology and evolution of cooperative breeding in birds. Cambridge University Press, Cambridge, UK, pp 197–209

    Google Scholar 

  • Withgott JH, Smith KG (1998) Brown-headed nuthatch (Sitta pusilla). In: Poole A, Gill F (eds) The birds of North America, No. 349. The Birds of North America, Inc, Philadelphia, PA, pp 1–23

    Google Scholar 

Download references

Acknowledgments

This research was partially supported by the Department of Zoology at the University of Florida. We thank the staff at TTRS for providing SEH with housing and permission to conduct field research on their property, J. Cox from TTRS for generous assistance with obtaining samples and E. Braun, K. Pause, and G. Ostrow for technical assistance.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sarah E. Haas.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haas, S.E., Smith, J.V., Kimball, R.T. et al. Isolation and characterization of polymorphic microsatellite markers for the brown-headed nuthatch (Sitta pusilla). Conserv Genet 10, 1393–1395 (2009). https://doi.org/10.1007/s10592-008-9703-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-008-9703-1

Keywords

Navigation