Abstract
Eleven dinucleotide and five tetranucleotide polymorphic microsatellite loci were presented for the Indo-Pacific humpback dolphin Sousa chinensis, a species categorized as ‘data deficiency’ in the IUCN Red List. These markers were developed to allow future population studies, such as characterization of population structure and genetic diversity that are important for the species’ conservation. The number of alleles ranged from 2 to 9 with observed heterozygosity ranging from 0.167 to 0.917, and expected heterozygosity ranging from 0.159 to 0.913. Test of these loci in five additional cetacean species found that 10–13 loci have successful cross-amplifications.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China grant nos 30670294 and 30470253, the Program for New Century Excellent Talents in University (NCET-07-0445), the Ministry of Education of China, the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP 20060319002), the Ministry of Education of China, and the Major Project for Basic Researches of Jiangsu Province Universities (07KJA18016). We thank Prof. Kaiya Zhou, Mr Anli Gao and Xin-Rong Xu for their assistance and contribution to collecting samples for many years.
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Chen, L., Yang, G. A set of polymorphic dinucleotide and tetranucleotide microsatellite markers for the Indo-Pacific humpback dolphin (Sousa chinensis) and cross-amplification in other cetacean species. Conserv Genet 10, 697–700 (2009). https://doi.org/10.1007/s10592-008-9618-x
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DOI: https://doi.org/10.1007/s10592-008-9618-x