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Isolation and charaterization of polymorphic microsatellite loci from so-iuy mullet (Mugil soiuy Basilewsky 1855)

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Abstract

The first set of polymorphic microsatellite markers were developed from so-iuy mullet (Mugil soiuy Basilewsky 1855). From a (GT)n-enriched genomic library, 53 microsatellites were selected for designing microsatellite primers, of which 36 gave working primer pairs. Ten of these loci were polymorphic in a test population of 24 individuals with alleles ranging from 3 to 9, and observed and expected heterozygosities from 0.2083 to 0.9167 and from 0.2651 to 0.8812, respectively. No significant linkage disequilibrium between pairs of loci was found, but two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy in Mullet.

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AcknowledgementS

We are grateful to Mr. Hao Ding for his assistance with this work, and this study was supported by State 863 High-technology R&D project of China (2006AA10A403), Taishan Scholar Project of Shandong Province, and scientific foundation of YSFRI, CAFS (2007-qn-13).

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Authors and Affiliations

  1. Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao, 266071, China

    Genbo Xu, Changwei Shao, Xiaolin Liao, Yongsheng Tian & Songlin Chen

  2. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Genbo Xu

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  1. Genbo Xu
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  2. Changwei Shao
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  3. Xiaolin Liao
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  4. Yongsheng Tian
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  5. Songlin Chen
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Correspondence to Songlin Chen.

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Genbo Xu and Changwei Shao Contributed equally.

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Xu, G., Shao, C., Liao, X. et al. Isolation and charaterization of polymorphic microsatellite loci from so-iuy mullet (Mugil soiuy Basilewsky 1855). Conserv Genet 10, 653–655 (2009). https://doi.org/10.1007/s10592-008-9602-5

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  • DOI: https://doi.org/10.1007/s10592-008-9602-5

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