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Microsatellite loci isolation in the endangered Gran Canarian blue chaffinch (Fringilla teydea polatzeki) and their utility in closely related taxa

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Abstract

The blue chaffinch, Fringilla teydea, is an endemic species of the Canary Islands. This species is formed by two subspecies: The Teneriffean blue chaffinch (F. t. teydea), and the endangered Gran Canarian blue chaffinch, (F. t. polatzeki). Here we report the isolation and characterization of nine tetranucleotide microsatellites (AAAG and AAAT) from the Gran Canarian subspecies, using an enrichment protocol. An average of 7.8 alleles per locus and an average observed heterozygosity of 0.773 were found (n = 28). The loci were tested for their ability to cross amplify in the Teneriffean subspecies and in the common chaffinch (Fringilla coelebs). These microsatellites will be used to manage a captive breeding programme for the endangered Gran Canarian subspecies.

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Acknowledgements

We would like to thank Monik Almeida, Davinia Carrillo, Omayra García, Vanesa González and Yessi Lara for laboratory assistance. The European Commission project LIFE98NAT/E/5354 and the Canary Islands Government Vice-council of the Environment co-financed this project.

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Correspondence to Nicolás M. Suárez.

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Suárez, N.M., González, A., Betancor, E. et al. Microsatellite loci isolation in the endangered Gran Canarian blue chaffinch (Fringilla teydea polatzeki) and their utility in closely related taxa. Conserv Genet 10, 581–583 (2009). https://doi.org/10.1007/s10592-008-9574-5

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  • DOI: https://doi.org/10.1007/s10592-008-9574-5

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