Skip to main content

Advertisement

SpringerLink
Log in

Microsatellite markers for the endangered razorback sucker, Xyrauchen texanus, are widely applicable to genetic studies of other catostomine fishes

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

We developed 10 polymorphic microsatellite markers for the federally endangered razorback sucker, Xyrauchen texanus. PCR optimization and cross-species amplification experiments indicated that these markers will be useful for analysis of fine-scale population structure in razorback and two other sucker species; the white sucker, Catostomus commersonii and the Rio Grande sucker, C. plebeius. Alleles at locus Xte4 did not overlap when compared between razorback and bluehead (C. discobolus) suckers and permits detection of F1 hybrids. These microsatellite markers appear widely applicable for identifying genetic consequences of population decline, hatchery rearing and release, and hybridization in razorback and other castostomine suckers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Harris PM, Mayden RL (2001) Phylogenetic relationships of major clades of Catostomidae (Teleostei: Cypriniformes) as inferred from mitochondrial SSU and LSU rDNA sequences. Mol Phylog Evol 20:225–237

    Article  CAS  Google Scholar 

  • McPhee MV, Turner TF (2004) No genetic evidence for hybridization between Rio Grande sucker, Catostomus plebeius, and the introduced white sucker, Catostomus commersoni, in the Rio Grande. Environ Biol Fish 71:85–93

    Article  Google Scholar 

  • Minckley WL, Deacon JE (1968) Southwestern fishes and the enigma of “endangered species”. Man’s invasion of deserts creates problems for native animals, especially for freshwater fishes. Science 159:1424–1432

    Article  PubMed  CAS  Google Scholar 

  • Minckley WL, Marsh PC, Brooks JE et al (1991) Management toward recovery of the razorback sucker. In: Minckley W, Deacon J (eds) Battle against extinction: native fish management in the American west. University of Arizona Press, Tempe AZ

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Smith GR (1992) Phylogeny and biogeography of the Catostomidae, freshwater fishes of North America and Asia. In: Mayden R (ed) Systematics, historical ecology, and North American freshwater fishes. Stanford University Press, Stanford CA

    Google Scholar 

Download references

Acknowledgements

We thank C. Secor, D. Alò and A. Sharp for technical assistance. Funding was provided by National Science Foundation (NSF) Awards DEB-0133233 (TFT) and DEB-9220683 (TED), the US Bureau of Reclamation (USBR) Agreement Number 01-FG-30-0023 (TED); and the Texas Agricultural Experiment Station under Project H-6703 (JRG). Razorback sucker tissues were obtained under state and federal permits, those from AZ-NV under US Fish and Wildlife Service subpermit number PRT-676811. Other fishes were collected under permit no. 3015 from the New Mexico Department of Game and Fish.

Author information

Authors and Affiliations

  1. Department of Biology and Museum of Southwestern Biology, University of New Mexico, Albuquerque, NM, 87131, USA

    Thomas F. Turner, Megan J. Osborne & Megan V. McPhee

  2. School of Life Sciences, Box, Arizona State University, Tempe, AZ, 85287, USA

    Thomas E. Dowling

  3. Flathead Lake Biological Station, University of Montana, Polson, MT, 59860, USA

    Megan V. McPhee

  4. Department of Zoology, University of Oklahoma, Norman, OK, 73019, USA

    Richard E. Broughton

  5. Center for Biosystematics and Biodiversity, Texas A&M University, College Station, TX, 77843, USA

    John R. Gold

Authors
  1. Thomas F. Turner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas E. Dowling
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Megan J. Osborne
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Megan V. McPhee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Richard E. Broughton
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John R. Gold
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Thomas F. Turner.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Turner, T.F., Dowling, T.E., Osborne, M.J. et al. Microsatellite markers for the endangered razorback sucker, Xyrauchen texanus, are widely applicable to genetic studies of other catostomine fishes. Conserv Genet 10, 551–553 (2009). https://doi.org/10.1007/s10592-008-9563-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-008-9563-8

Keywords

Search

Navigation