Abstract
We report on the identification and characterization of six EST-linked simple sequence repeats (EST-SSRs) and chloroplast SSRs (cpSSRs) in endangered Cycas hainanensis. The number of alleles ranged from two to eight for EST-SSRs, two to three for cpSSRs. Observed and expected heterozygosities ranged from 0.042 to 0.417 and 0.042 to 0.811 for EST-SSRs, respectively. Expected heterozygosities ranged from 0.156 to 0.457 for cpSSRs. All these markers gave successful cross-species amplification in C. fairylakea. These markers will allow analyses of the baseline genetic variability and population structure of C. hainanensis to provide strategies for effective conservation and management.
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References
Barrett SCH, Kohn JR (1991) Genetic and evolutionary consequences of small population size in plants: implications for conservation. In: Falk DA, Holsinger KE (eds) Genetics and conservation of rare plants. Oxford University Press, New York, pp 3–30
Lewis PO, Zaykin D (2001) Genetic data analysis: computer program for the analysis of allelic data. Version 1.0 (d16c). Free program distributed by the authors over the internet from http://www.lewis.eeb.uconn.edu/lewishome/software.html
Liu N, Fan H-M (1999) The comparison of populations between two cycads on Hainan Island. In: Chen CJ (eds) Biology and conservation of cycads-proceeding of the fourth international conference on cycad biology, Panzhihua, China. International Academic Publishers, Beijing, pp 63–67
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86(3):248–249
Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S (2001) Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Res 11:1441–1452
Timmis JN, Scott NS (1983) Sequence homology between spinach nuclear and chloroplast genomes. Nature 305:65–67
Ying T-S, Cheng M-L, Chang H-C (eds) (2004) Atlas of the gymnosperms of China. China Science and Technology press, Beijing
Acknowledgements
We thank Peng Zeqiu and Jian shuguang for helping collect/identify samples. This work was supported by National Basic Research Program of China (973 Program) (No. 2007CB411600), the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-YW-Z-023) and Science Utility Project of Guangdong Province (No. 2005B60301001).
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The experiments were carried out in South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, P.R. China.
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Wang, ZF., Ye, WH., Cao, HL. et al. Identification and characterization of EST-SSRs and cpSSRs in endangered Cycas hainanensis . Conserv Genet 9, 1079–1081 (2008). https://doi.org/10.1007/s10592-007-9461-5
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DOI: https://doi.org/10.1007/s10592-007-9461-5