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Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica

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Abstract

We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.

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Acknowledgements

We are grateful to D. Little and D. Stevenson for collecting all but Cycas micronesica samples, and the Florida Montgomery Botanical Center for kindly providing them. We also thank Venkatesh Alapati and Helen Ma for their contribution in the processing of the samples. This research was supported by the U.S. Department of Agriculture CSREES Award 2006-34135-17141.

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Authors and Affiliations

  1. Genomics, The New York Botanical Garden, 200th St. and Kazimiroff Blvd, Bronx, NY, 10458, USA

    Angélica Cibrián-Jaramillo & Eric D. Brenner

  2. Sackler Institute for Comparative Genomics, American Museum of Natural History, Central Park West at 79th St, New York, NY, 10024, USA

    Angélica Cibrián-Jaramillo & Rob DeSalle

  3. Western Pacific Tropical Research Center, College of Natural and Applied Sciences, University of Guam, UOG Station, Mangilao, Guam, 96923, USA

    Thomas E. Marler

Authors
  1. Angélica Cibrián-Jaramillo
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  2. Thomas E. Marler
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  3. Rob DeSalle
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  4. Eric D. Brenner
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Correspondence to Angélica Cibrián-Jaramillo.

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Cibrián-Jaramillo, A., Marler, T.E., DeSalle, R. et al. Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica . Conserv Genet 9, 1051–1054 (2008). https://doi.org/10.1007/s10592-007-9447-3

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  • DOI: https://doi.org/10.1007/s10592-007-9447-3

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