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Isolation and characterization of microsatellite loci in the endangered freshwater fish Pararasbora moltrechti (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

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Abstract

Pararasbora moltrechti (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the central Taiwan. In order to undertake a conservation program for this species in Taiwan, it is essential to evaluate its genetic diversity and population structure. For this purpose, we described the development of eight microsatellite loci in P. moltrechti for genetic studies. These new molecular markers were tested in 36 individuals of the rare species. The number of alleles per locus ranged from 8 to 14. As the expected heterozygosity varied from 0.770 to 0.932, all microsatellite loci, however, were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency.

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Acknowledgments

The study was supported by a grant of the Council of Agriculture, Taiwan.

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Authors and Affiliations

  1. Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan

    Hung-Du Lin, Fang-Jiao Lin, Chung-Jian Lin & Tzen-Yuh Chiang

  2. Taiwan Endemic Species Research Institute, ChiChi, Nantou, 552, Taiwan

    Teh-Wang Lee

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  1. Hung-Du Lin
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  2. Teh-Wang Lee
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  3. Fang-Jiao Lin
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  4. Chung-Jian Lin
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  5. Tzen-Yuh Chiang
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Corresponding author

Correspondence to Tzen-Yuh Chiang.

Additional information

Hung-Du Lin and Teh-Wang Lee contributed equally.

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Lin, HD., Lee, TW., Lin, FJ. et al. Isolation and characterization of microsatellite loci in the endangered freshwater fish Pararasbora moltrechti (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet 9, 945–947 (2008). https://doi.org/10.1007/s10592-007-9365-4

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  • DOI: https://doi.org/10.1007/s10592-007-9365-4

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