Skip to main content
SpringerLink
Log in

Isolation and characterization of polymorphic microsatellite loci in Ardisia crenata (Myrsinaceae)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Ardisia crenata, an evergreen shrub native to East Asia, has been a serious invasive plant to the southeastern USA. Here 13 polymorphic microsatellite loci were isolated and characterized from an enrichment genomic library of A. crenata. The average allele number of these microsatellites was four per locus, ranging from two to seven. The ranges of observed and expected heterozygosity were 0.000–1.000 and 0.239–0.789, respectively. These microsatellite markers will be useful for investigating population genetics and reproductive ecology of A. crenata.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer 3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, New Jersey, pp 365–386

    Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (30530160) and the Natural Science Foundation of Guangdong Province, China (05200701).

Author information

Authors and Affiliations

  1. South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou, 510650, China

    Lan Hong, Hongyu Niu, Hao Shen, Wanhui Ye & Honglin Cao

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, China

    Lan Hong

Authors
  1. Lan Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongyu Niu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hao Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wanhui Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Honglin Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wanhui Ye.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hong, L., Niu, H., Shen, H. et al. Isolation and characterization of polymorphic microsatellite loci in Ardisia crenata (Myrsinaceae). Conserv Genet 9, 491–494 (2008). https://doi.org/10.1007/s10592-007-9360-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-007-9360-9

Keywords

Search

Navigation