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Periostin, a stroma-associated protein, correlates with tumor invasiveness and progression in nasopharyngeal carcinoma

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Abstract

Recently, the tumor microenvironment is increasingly recognized as playing an important role in cancer proliferation, invasion, and metastasis. To screen stroma-associated proteins involved in nasopharyngeal carcinoma (NPC) carcinogenesis, laser capture microdissection (LCM) and quantitative proteomic analysis were employed to assess different protein expression of the stroma between NPC and normal nasopharyngeal mucosa (NNM). In this study, periostin was identified to be significantly up-regulated in NPC stroma compared with NNM stroma and the result was further confirmed by Western blotting. Immunohistochemistry showed that over-expression of periostin was frequently observed in the stroma of NPC and matched lymph node metastases (LNM) compared with the stroma of NNM. Statistical analysis showed over-expression of periostin was significantly associated with advanced clinical stage (P < 0.001) and lymph node metastasis (P < 0.001) and decreased overall survival (P < 0.001) in NPC. Cox regression analysis indicated over-expression of periostin was an independent prognostic factor. Furthermore, ectopic expression of periostin was used to examine its effect on invasiveness of NPC cell in vitro and the result showed that periostin was able to promote invasiveness of NPC cell. In conclusion, periostin expression is correlated with tumor stage, lymph node metastasis, and patient survival. Periostin is a potential biomarker for the differentiation and prognosis of NPC, and it might play an important role in the progression of NPC.

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Abbreviations

NPC:

Nasopharyngeal carcinoma

NNM:

Normal nasopharyngeal mucosa

LCM:

Laser capture microdissection

LNM:

Lymph node metastases

TS:

Tumor stroma

NS:

Normal stroma

2D-DIGE:

Two-dimensional difference gel electrophoresis

MS:

Mass spectrometry

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Acknowledgments

This research was supported by grants from National Natural Sciences Foundation of China (Grant No. 81072199, 30973289), Scientific Research Foundation for Dr. of University of South China (2010XQD38), National Basic Research Program of China (2011CB910704), Science and Technology Research Program of Hunan Province in China (2011TT2015). We gratefully acknowledge Professor Rong Shao (University of Massachusetts, USA) for providing pCMV-neo-periostin plasmid.

Conflict of interest

No conflicts of interest are declared.

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Authors and Affiliations

  1. Department of Histology and Embryology, School of Medicine, University of South China, 421001, Hengyang, China

    Meixiang Li, Yuanjie Xie & Jinfeng Shi

  2. Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 87# Xiangya Road, 410008, Changsha, Hunan, China

    Cui Li, Guoqing Li, Maoyu Li, Pengfei Zhang, Fang Peng, Zhiqiang Xiao & Zhuchu Chen

  3. Department of Oncology, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, China

    Danjuan Li

  4. Cancer Research Institute, Xiangya School of Medicine, Central South University, 410078, Changsha, China

    Yongjun Guan

  5. School of Life Science and Technology, University of South China, 421001, Hengyang, China

    Guoqing Li

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  1. Meixiang Li
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  2. Cui Li
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Correspondence to Zhuchu Chen.

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Li, M., Li, C., Li, D. et al. Periostin, a stroma-associated protein, correlates with tumor invasiveness and progression in nasopharyngeal carcinoma. Clin Exp Metastasis 29, 865–877 (2012). https://doi.org/10.1007/s10585-012-9465-5

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  • DOI: https://doi.org/10.1007/s10585-012-9465-5

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