Abstract
Surgical therapy of peritoneal surface malignancy from colorectal origin in combination with Hyperthermic Intraoperative Peritoneal Chemotherapy (HIPEC) has now become an established treatment approach in very few specialised centres. A peritonectomy procedure is possible to perform with additional HIPEC in patients. An experimental model to simulate peritonectomy procedure and HIPEC does not exist so far in rats. Nevertheless, animal models seem to be very important for evaluation of new therapeutic opportunities and toxicity of different multimodal therapies. In a first step we analysed the surgical tumour debulking of peritoneal surface malignancy in rats. A peritoneal surface malignancy from colonic origin was induced in 75 male BD IX rats. Twenty one days after induction of peritoneal surface malignancy rats were randomised and animals intend to create an operation with surgical tumour debulking. There was no tumour growth in two animals. The aim of the peritonectomy procedure was the complete tumour reduction. In this study the results of the surgical approach will be described. A complete tumour reduction (R0) was achieved in 34 animals. In 39 rats a macroscopic tumour deposit was left behind (R2). The intraoperative experimental Peritoneal Cancer Index (ePCI) was used to describe tumour weight and number of tumour inoculations. Both parameters were found to be dependent factors of complete tumour reduction. Six animals died due to therapeutical interventions. Surgical tumour debulking in rats with peritoneal surface malignancy is possible with high reliability and a low mortality rate. This animal model could be an important step for investigation of multimodal treatment options and toxicity in treatment regimens of peritoneal surface malignancy.
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Sugarbaker PH (1995) Peritonectomy procedures. Ann Surg 221(1):29–42
Verwaal VJ, van Ruth S, de Bree E et al (2003) Randomized trial of cytoreduction and hyperthermic intra-peritoneal chemotherapy versus systemic chemotherapy and palliative surgery in patients with peritoneal carcinomatosis of colorectal cancer. J Clin Oncol 12:3737–3743
Glehen O, Kwiatkowski F, Sugarbaker PH et al (2004) Cytoreductive surgery combined with perioperative intraperitoneal chemotherapy for the management of peritoneal carcinomatosis from colorectal cancer: a multi-institutional study. J Clin Oncol 22(16):3284–3292
Ismail M, Henklein P, Huang X et al (2006) Identification of HIV-1 Tat peptides for future therapeutic angiogenesis. Eur J Haematol 77(2):157–165
Steller EP, Ottow RT, Matthews W et al (1985) Recombinant interleukin-2 and adoptively transferred lymphokine-activated killer cells in the treatment of experimental peritoneal carcinomatosis. Surg Forum 36:390–392
Brodsky JT, Cohen AM (1991) Peritoneal seeding following potentially curative resection of colonic carcinoma: implications of adjuvant therapy. Dis Colon Rectum 34(8):723–727
Pelz JOW, Doerfer J, Hohenberger W et al (2005) A new survival model for intraperitoneal chemotherapy (HIPEC) in tumor-bearing rats in the treatment of peritoneal carcinomatosis. BMC Cancer 5:65
Los G, Siminia P, Wondergerem J et al (1991) Optimisation of intraperitoneal cisplatin therapy with regional hyperthermia in rats. Eur J Cancer 27:472–477
Hribaschek A, Pross M, Ridwelski K et al (2004) Five cytostatic substances in animal studies for prevention and treatment of experimentally induced peritoneal carcinomatosis. Zentralbl Chir 129(4):328–334
Veenhuizen RB, Marijnissen JP, Kenemans P et al (1996) Intraperitoneal photodynamic therapy of the rat CC531 adenocarcinoma. Br J Cancer 73(11):1387–1392
Balague C, Braumann C, Fuhrer K et al (2001) Validation of a new experimental model of colon cancer. Surg Endosc 15:833–836
Ordemann J, Hoeflich C, Braumann C et al (2005) Impact of pneumoperitoneum on expression of E-cadherin, CD44v6 and CD54 (ICAM-1) on HT-29 colon-carcinoma cells. Zentralbl Chir 130(5):405–409
Braumann C, Ordemann J, Kilian M et al (2003) Local and systemic chemotherapy with taurolidine and taurolidine/heparin in colon cancer-bearing rats undergoing laparotomy. Clin Exp Metastasis 20(5):387–394
Glehen O, Mohamed F, Sugarbaker PH (2004) Incomplete cytoreduction in 174 patients with peritoneal carcinomatosis from appendiceal malignancy. Ann Surg 240(2):278–285
Elias D, Blot F, El Otmany A et al (2001) Curative treatment of peritoneal carcinomatosis arising from colorectal cancer by complete resection and intraperitoneal chemotherapy. Cancer 92(1):71–76
Boni L, Benevento A, Dionigi G et al (2005) Injection of colorectal cancer cells in mesenteric and antimesenteric sides of the colon results in different patterns of metastatic diffusion: an experimental study in rats. World J Surg Oncol 3:69
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Hartmann, J., Kilian, M., Atanassov, V. et al. First surgical tumour reduction of peritoneal surface malignancy in a rat’s model. Clin Exp Metastasis 25, 445–449 (2008). https://doi.org/10.1007/s10585-008-9150-x
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DOI: https://doi.org/10.1007/s10585-008-9150-x