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A surgically induced hypoxic environment causes changes in the metastatic behaviour of tumours in vitro

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Abstract

The use of laparoscopic techniques for curative resections of malignant tumours has been under scrutiny. The potential benefits to the patient in the form of earlier recovery and less immune paresis are countered by the reports of increased tumour recurrence. The biological sequelae of the hypoxic laparoscopic environment on tumour cells is unknown. Components of the metastatic cascade were evaluated under in vitro laparoscopic conditions using a human colonic adenocarcinoma cell line (SW1222). Exposure to the laparoscopic gases carbon dioxide and helium for 4 h, comparable to the duration of a laparoscopic colorectal resection, had no effect on cell viability. A cellular hypoxic insult was demonstrated by the induction of hypoxia inducible factor 1α (HIF-1α). Exposure also resulted in significant reduction in homotypic adhesion as well as to a variety of extracellular matrix components. These effects were recoverable under re-oxygenation. The changes were reflected at the molecular level by significant down regulation of adhesion molecules known to be involved in tumour progression (E-cadherin, CD44 and β1 sub-unit). Modulation of adherence has significant implications for laparoscopic oncological surgery, demonstrating that tumours become potentially more friable and easier to disseminate in surgeons who are less experienced or where instrumentation is sub-optimal.

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Authors and Affiliations

  1. Department of Biosurgery & Surgical Technology, Division of Surgery, Oncology, Reproductive Biology and Intensive Care, Imperial College London, St Mary’s Hospital, 10th Floor QEQM wing, London, W2 1NY, UK

    P. A. Paraskeva, P. F. Ridgway, S. Olsen, C. Isacke, D. H. Peck & A. W. Darzi

  2. Department of Biology, Imperial College London, South Kensington, London, SW7, UK

    C. Isacke

Authors
  1. P. A. Paraskeva
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  2. P. F. Ridgway
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  3. S. Olsen
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  4. C. Isacke
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  5. D. H. Peck
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  6. A. W. Darzi
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Corresponding author

Correspondence to P. A. Paraskeva.

Additional information

This project was jointly supervised by D.H. Peck and A.W. Darzi

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Paraskeva, P.A., Ridgway, P.F., Olsen, S. et al. A surgically induced hypoxic environment causes changes in the metastatic behaviour of tumours in vitro. Clin Exp Metastasis 23, 149–157 (2006). https://doi.org/10.1007/s10585-006-9028-8

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  • DOI: https://doi.org/10.1007/s10585-006-9028-8

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