Abstract
Matrix metalloproteinases (MMPs), in particular the gelatinases MMP2 and MMP9, are important mediators of tumour invasion and metastasis. We examined whether plasma gelatinase levels could predict lymph node metastasis in breast cancer patients. Further, we investigated the relationship of plasma gelatinase levels with Her2/neu expression, recently acknowledged as an important prognostic factor for recurrence, and with various clinicopathological factors. Preoperative plasma samples from 81 breast cancer patients were collected. Total and active gelatinase levels were measured by enzyme immunoassays and activity assays, respectively. Neither total nor active plasma MMP2 levels correlated with nodal status or with any of the classical clinicopathological factors including histological tumour type, tumour size and grade and hormone receptor status. Patients with Her2/neu overexpressing tumours showed an increase of 27% (P=0.007) in plasma MMP2 activity, but not in total MMP2, compared with patients without overexpression. MMP9 levels, total and active, did not correlate with any of the investigated variables. In contrast to MMP9, total MMP2 levels correlated significantly with active MMP2 levels. In summary, total and active plasma gelatinase levels failed to identify high risk for axillary lymph node metastasis. Active plasma MMP2 was significantly increased in patients with Her2/neu overexpressing tumours, suggesting a role for Her2/neu in the signalling pathways of MMP2 activation in carcinogenesis. However, this increase was too small to be of clinical use. Furthermore, no relationship was found between plasma gelatinase levels, total or active, and any of the clinicopathological prognostic factors.
Similar content being viewed by others
Abbreviations
- AP-2:
-
transcription factor activator protein
- APMA:
-
p-aminophenylmercuric acetate
- CEA:
-
carcinoembryonic antigen
- CA15-3:
-
cancer antigen 15-3
- DAB:
-
3,3-diaminobenzidine
- ER:
-
oestrogen receptor
- FISH:
-
fluorescence in situ hybridization
- Her2/neu:
-
human epidermal growth factor receptor 2
- MMP:
-
matrix metalloproteinase
- MT-MMPs:
-
membrane type matrix metalloproteinase
- PgR:
-
progesteron receptor
- sELISA:
-
sandwich enzyme-linked immunoassay
- TIMPs:
-
tissue inhibitor matrix metalloproteinase
References
MP Coleman G Gatta A Verdecchia et al. (2003) ArticleTitleEUROCARE-3 summary: cancer survival in Europe at the end of the 20th century Ann Oncol 14 IssueIDSuppl 5 v128–v149 Occurrence Handle14684503
WL McGuire GM Clark (1992) ArticleTitlePrognostic factors and treatment decisions in axillary-node-negative breast cancer N Engl J Med 326 IssueID26 1756–61 Occurrence Handle1594018 Occurrence Handle1:STN:280:By2B2sfjvFA%3D
M Egeblad Z Werb (2002) ArticleTitleNew functions for the matrix metalloproteinases in cancer progression Nat Rev Cancer 2 IssueID3 161–74 Occurrence Handle10.1038/nrc745 Occurrence Handle11990853 Occurrence Handle1:CAS:528:DC%2BD38Xis1KktL8%3D
MA Lafleur MM Handsley DR Edwards (2003) ArticleTitleMetalloproteinases and their inhibitors in angiogenesis Expert Rev Mol Med 2003 1–39 Occurrence Handle14585170
G La Rocca I Pucci-Minafra A Marrazzo et al. (2004) ArticleTitleZymographic detection and clinical correlations of MMP-2 and MMP-9 in breast cancer sera Br J Cancer 90 IssueID7 1414–21 Occurrence Handle15054465 Occurrence Handle1:CAS:528:DC%2BD2cXis1aitbY%3D
A Talvensaari-Mattila P Paakko M Hoyhtya et al. (1998) ArticleTitleMatrix metalloproteinase-2 immunoreactive protein: a marker of aggressiveness in breast carcinoma Cancer 83 IssueID6 1153–62 Occurrence Handle10.1002/(SICI)1097-0142(19980915)83:6<1153::AID-CNCR14>3.0.CO;2-4 Occurrence Handle9740080 Occurrence Handle1:CAS:528:DyaK1cXmtlegsrg%3D
A Scorilas A Karameris N Arnogiannaki et al. (2001) ArticleTitleOverexpression of matrix-metalloproteinase-9 in human breast cancer: a potential favourable indicator in node-negative patients Br J Cancer 84 IssueID11 1488–96 Occurrence Handle10.1054/bjoc.2001.1810 Occurrence Handle11384099 Occurrence Handle1:STN:280:DC%2BD3MzhtFGmtg%3D%3D
A Talvensaari-Mattila P Paakko T Turpeenniemi-Hujanen (1999) ArticleTitleMMP-2 positivity and age less than 40 years increases the risk for recurrence in premenopausal patients with node-positive breast carcinoma Breast Cancer Res Treat 58 IssueID3 287–93 Occurrence Handle10.1023/A:1006326513176 Occurrence Handle10718490 Occurrence Handle1:STN:280:DC%2BD3c7otFCiug%3D%3D
A Talvensaari-Mattila P Paakko G Blanco-Sequeiros T Turpeenniemi-Hujanen (2001) ArticleTitleMatrix metalloproteinase-2 (MMP-2) is associated with the risk for a relapse in postmenopausal patients with node-positive breast carcinoma treated with antiestrogen adjuvant therapy Breast Cancer Res Treat 65 IssueID1 55–61 Occurrence Handle10.1023/A:1006458601568 Occurrence Handle11245340 Occurrence Handle1:CAS:528:DC%2BD3MXhvFektbg%3D
A Talvensaari-Mattila P Paakko T Turpeenniemi-Hujanen (2003) ArticleTitleMatrix metalloproteinase-2 (MMP-2) is associated with survival in breast carcinoma Br J Cancer 89 IssueID7 1270–5 Occurrence Handle10.1038/sj.bjc.6601238 Occurrence Handle14520459 Occurrence Handle1:CAS:528:DC%2BD3sXns1Cmur0%3D
SM Sheen-Chen HS Chen HL Eng et al. (2001) ArticleTitleSerum levels of matrix metalloproteinase 2 in patients with breast cancer Cancer Lett 173 IssueID1 79–82 Occurrence Handle10.1016/S0304-3835(01)00657-7 Occurrence Handle11578812 Occurrence Handle1:CAS:528:DC%2BD3MXntV2jsbc%3D
S Zucker RM Lysik MH Zarrabi U Moll (1993) ArticleTitleM(r) 92,000 type IV collagenase is increased in plasma of patients with colon cancer and breast cancer Cancer Res 53 IssueID1 140–6 Occurrence Handle8416738 Occurrence Handle1:STN:280:ByyC3crktVU%3D
S Zucker M Hymowitz C Conner et al. (1999) ArticleTitleMeasurement of matrix metalloproteinases and tissue inhibitors of metalloproteinases in blood and tissues. Clinical and experimental applications Ann N Y Acad Sci 878 212–27 Occurrence Handle10415733 Occurrence Handle1:CAS:528:DyaK1MXkslemsrc%3D
S Leppa T Saarto L Vehmanen et al. (2004) ArticleTitleA high serum matrix metalloproteinase-2 level is associated with an adverse prognosis in node-positive breast carcinoma Clin Cancer Res 10 IssueID3 1057–63 Occurrence Handle14871985
SM Ranuncolo E Armanasco C Cresta et al. (2003) ArticleTitlePlasma MMP-9 (92 kDa-MMP) activity is useful in the follow-up and in the assessment of prognosis in breast cancer patients Int J Cancer 106 IssueID5 745–51 Occurrence Handle10.1002/ijc.11288 Occurrence Handle12866035 Occurrence Handle1:CAS:528:DC%2BD3sXms1Wjsrw%3D
KM Keller JM Keller K Kuhn (1986) ArticleTitleThe C-terminus of type I collagen is a major binding site for heparin Biochim Biophys Acta 882 IssueID1 1–5 Occurrence Handle3011107 Occurrence Handle1:CAS:528:DyaL28XktVansLg%3D
GS Makowski ML Ramsby (2003) ArticleTitleUse of citrate to minimize neutrophil matrix metalloproteinase-9 in human plasma Anal Biochem 322 IssueID2 283–6 Occurrence Handle10.1016/j.ab.2003.07.030 Occurrence Handle14596841 Occurrence Handle1:CAS:528:DC%2BD3sXosVygsb4%3D
UM Wallon CM Overall (1997) ArticleTitleThe hemopexin-like domain (C domain) of human gelatinase A (matrix metalloproteinase-2) requires Ca2+ for fibronectin and heparin binding. Binding properties of recombinant gelatinase A C domain to extracellular matrix and basement membrane components J Biol Chem 272 IssueID11 7473–81 Occurrence Handle9054449 Occurrence Handle1:CAS:528:DyaK2sXhvFKhsbs%3D
K Jung M Lein C Laube R Lichtinghagen (2001) ArticleTitleBlood specimen collection methods influence the concentration and the diagnostic validity of matrix metalloproteinase 9 in blood Clin Chim Acta 314 IssueID1–2 241–4 Occurrence Handle11718702 Occurrence Handle1:CAS:528:DC%2BD3MXosFKktrg%3D
R Hanemaaijer JH Verheijen TM Maguire et al. (2000) ArticleTitleIncreased gelatinase-A and gelatinase-B activities in malignant vs. benign breast tumors Int J Cancer 86 IssueID2 204–7 Occurrence Handle10.1002/(SICI)1097-0215(20000415)86:2<204::AID-IJC9>3.0.CO;2-6 Occurrence Handle10738247 Occurrence Handle1:CAS:528:DC%2BD3cXis1CksLY%3D
RB Dickson ME Lippman (1995) ArticleTitleGrowth factors in breast cancer Endocr Rev 16 IssueID5 559–89 Occurrence Handle10.1210/er.16.5.559 Occurrence Handle8529572 Occurrence Handle1:CAS:528:DyaK2MXpsVChtb4%3D
G Konecny M Untch J Arboleda et al. (2001) ArticleTitleHer-2/neu and urokinase-type plasminogen activator and its inhibitor in breast cancer Clin Cancer Res 7 IssueID8 2448–57 Occurrence Handle11489825 Occurrence Handle1:CAS:528:DC%2BD3MXmsVOmtLk%3D
F Bertucci N Borie C Ginestier et al. (2004) ArticleTitleIdentification and validation of an ERBB2 gene expression signature in breast cancers Oncogene 23 IssueID14 2564–75 Occurrence Handle10.1038/sj.onc.1207361 Occurrence Handle14743203 Occurrence Handle1:CAS:528:DC%2BD2cXis1Gmtrw%3D
D Giunciuglio M Culty G Fassina et al. (1995) ArticleTitleInvasive phenotype of MCF10A cells overexpressing c-Ha-ras and c-erbB-2 oncogenes Int J Cancer 63 IssueID6 815–22 Occurrence Handle8847140 Occurrence Handle1:CAS:528:DyaK28Xpt12qtA%3D%3D
P O’Charoenrat P Rhys-Evans WJ Court et al. (1999) ArticleTitleDifferential modulation of proliferation, matrix metalloproteinase expression and invasion of human head and neck squamous carcinoma cells by c-erbB ligands Clin Exp Metastasis 17 IssueID7 631–9 Occurrence Handle1:CAS:528:DC%2BD3cXksl2lsrg%3D
A Roetger A Merschjann T Dittmar et al. (1998) ArticleTitleSelection of potentially metastatic subpopulations expressing c-erbB-2 from breast cancer tissue by use of an extravasation model Am J Pathol 153 IssueID6 1797–806 Occurrence Handle9846970 Occurrence Handle1:CAS:528:DyaK1cXotV2ku7g%3D
M Tan J Yao D Yu (1997) ArticleTitleOverexpression of the c-erbB-2 gene enhanced intrinsic metastasis potential in human breast cancer cells without increasing their transformation abilities Cancer Res 57 IssueID6 1199–205 Occurrence Handle9067293 Occurrence Handle1:CAS:528:DyaK2sXhvFOitr8%3D
FJ Xu S Stack C Boyer et al. (1997) ArticleTitleHeregulin and agonistic anti-p185(c-erbB2) antibodies inhibit proliferation but increase invasiveness of breast cancer cells that overexpress p185(c-erbB2): increased invasiveness may contribute to poor prognosis Clin Cancer Res 3 IssueID9 1629–34 Occurrence Handle9815853 Occurrence Handle1:CAS:528:DyaK2sXmt1yht7g%3D
D Yu SS Wang KM Dulski et al. (1994) ArticleTitlec-erbB-2/neu overexpression enhances metastatic potential of human lung cancer cells by induction of metastasis-associated properties Cancer Res 54 IssueID12 3260–6 Occurrence Handle7911396 Occurrence Handle1:CAS:528:DyaK2cXksFCqsr0%3D
JM Pellikainen KM Ropponen VV Kataja et al. (2004) ArticleTitleExpression of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer with a special reference to activator protein-2, HER2, and prognosis Clin Cancer Res 10 IssueID22 7621–8 Occurrence Handle15569994 Occurrence Handle1:CAS:528:DC%2BD2cXhtVanurzI
J Westermarck VM Kahari (1999) ArticleTitleRegulation of matrix metalloproteinase expression in tumor invasion FASEB J 13 IssueID8 781–92 Occurrence Handle10224222 Occurrence Handle1:CAS:528:DyaK1MXjtFGksrs%3D
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Decock, J., Hendrickx, W., Wildiers, H. et al. Plasma Gelatinase Levels in Patients with Primary Breast Cancer in Relation to Axillary Lymph Node Status, Her2/neu Expression and other Clinicopathological Variables. Clin Exp Metastasis 22, 495–502 (2005). https://doi.org/10.1007/s10585-005-3992-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10585-005-3992-2