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Mouse Colon Carcinoma Cells Established for High Incidence of Experimental Hepatic Metastasis Exhibit Accelerated and Anchorage-Independent Growth

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Abstract

Highly metastatic variants of mouse colon 38 colon carcinoma cells were established by repeated selection in vivo for liver metastasis and designated as SL4 cells. The SL4 cells formed colonies in the liver of 100% of syngenic mice when injected intrasplenically, while the incidence of liver metastasis was 27% of mice injected with parental cells. The weight of livers, which is an indicator of experimental hepatic metastasis formation, was significantly higher after intrasplenic injection and subsequent splenoctomy with SL4 cells than colon 38 cells. The incidence of hepatic metastasis after intracecal injection of SL4 cells was significantly higher than that of colon 38 cells. The SL4 cells were tested in vitro for their properties. Differences were not detected in the motility and invasive behavior between colon 38 cells and SL4 cells. SL4 cells showed a higher proliferation rate than colon 38 cells under adherent conditions. SL4 cells maintained a capacity to proliferate under non-adherent conditions whereas parental cells did not. SL4 cells should be a useful tool to study the mechanism of hepatic metastasis of colon carcinoma cells and to develop methods to prevent hepatic metastasis.

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Abbreviations

DMEM/F12:

a 1:1 mixture of Dulbecco’s modified Eagle’s medium and Ham’s F-12 medium

PBS:

phosphate buffered saline

polyHEMA:

poly(2-hydroxyethylmethacrylate)

TRITC-phalloidin:

tetramethylrhodamine isothiocyanate-conjugated phalloidin

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Authors and Affiliations

  1. Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan

    Megumi Morimoto-Tomita, Yoshimi Ohashi, Azusa Matsubara, Makoto Tsuiji & Tatsuro Irimura

  2. Department of Anatomy, University of California, San Francisco, California, USA

    Megumi Morimoto-Tomita

Authors
  1. Megumi Morimoto-Tomita
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  2. Yoshimi Ohashi
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  3. Azusa Matsubara
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  4. Makoto Tsuiji
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  5. Tatsuro Irimura
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Correspondence to Tatsuro Irimura.

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Morimoto-Tomita, M., Ohashi, Y., Matsubara, A. et al. Mouse Colon Carcinoma Cells Established for High Incidence of Experimental Hepatic Metastasis Exhibit Accelerated and Anchorage-Independent Growth. Clin Exp Metastasis 22, 513–521 (2005). https://doi.org/10.1007/s10585-005-3585-0

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  • DOI: https://doi.org/10.1007/s10585-005-3585-0

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