Abstract
This study compared cortisol concentrations yielded using three saliva collection methods (passive drool, salivette, and sorbette) in both in vitro and in vivo conditions, as well as method acceptability for a sample of children (n = 39) with High Functioning Autism Spectrum Disorders. No cortisol concentration differences were observed between passive and sorbette samples obtained in vitro or in vivo. The salivette derived concentration was lower than the other two methods for the in vitro derived comparisons but did not differ from the other methods when collected in vivo. Cross-day comparison for the salivettes was also found to differ significantly, whereas the cross-day comparisons did not differ for the passive method or the sorbette method. Overall, passive drool and sorbettes were found to produce similar and stable readings of cortisol, whereas the salivette yielded unstable and variable concentrations. Ratings suggested that the children generally perceived all methods as acceptable.
Similar content being viewed by others
References
American Psychiatric Association (2000) Diagnostic and statistical manual of mental disorders 4th edition (DSM-IV-TR). American Psychiatric Association, Washington, DC
Baron MG, Lipsitt LP, Groden J, Groden G (2006) Introduction. In: Baron MG, Groden J, Groden G, Lipsitt LP (eds) Stress and coping in autism. Oxford University Press, New York, pp 3–11
Tsai L (2006) Diagnosis and treatment of anxiety disorders in individuals with autism spectrum disorder. In: Baron MG, Groden J, Groden G, Lipsitt LP (eds) Stress and coping in autism. Oxford University Press, New York, pp 388–440
Weisbrot DM, Gadow KD, DeVincent CJ, Pomeroy J (2005) The presentation of anxiety in children with pervasive developmental disorders. J Child Adol Psychop 15(3):477–496
Gadow KD, DeVincent CJ, Pomeroy J, Azizian A (2005) Comparison of DSM-IV symptoms in elementary school-age children with PDD versus clinic and community samples. Autism 9(4):392–415
Gillott A, Furniss F, Walter A (2001) Anxiety in high-functioning children with autism. Autism 5(3):277–286
Lopata C, Volker MA, Putnam SK, Thomeer ML, Nida RE (2008) Effect of social familiarity on salivary cortisol and self-reports of social anxiety and stress in children with high functioning autism spectrum disorders. J Autism Dev Disord 38:1866–1877
Morgan K (2006) Is autism a stress disorder? What studies of nonautistic populations can tell us. In: Baron MG, Groden J, Groden G, Lipsitt LP (eds) Stress and coping in autism. Oxford University Press, New York, pp 129–182
Church C, Alisanski S, Amanullah S (2000) The social, behavioral, and academic experiences of children with Asperger syndrome. Focus Autism Other Dev Disabil 15(1):2–20
Groden J, Baron MG, Groden G (2006) Assessment and coping strategies. In: Baron MG, Groden J, Groden G, Lipsitt LP (eds) Stress and coping in autism. Oxford University Press, New York, pp 15–41
Lopata C, Gitman K, Toomey JA, Volker MA, Nida RE, Thomeer ML, McDonald CA (2009) Experimental study examining the effect of social familiarity on behavioral manifestations of stress and anxiety in children with high-functioning autism spectrum disorders. In: Heidenreich P, Pruter I (eds) Handbook of stress: causes, effects, and control. Nova Science, Hauppauge, pp 347–365
Romanczyk RG, Gillis JM (2006) Autism and the physiology of stress and anxiety. In: Baron MG, Groden J, Groden G, Lipsitt LP (eds) Stress and coping in autism. Oxford University Press, New York, pp 183–204
Baron MG, Lipsitt LP, Goodwin MS (2006) Scientific foundations for research and practice. In: Baron MG, Groden J, Groden G, Lipsitt LP (eds) Stress and coping in autism. Oxford University Press, New York, pp 42–67
Hill E, Berthoz S, Frith U (2004) Brief report: cognitive processing of own emotions in individuals with autistic spectrum disorder and in their relatives. J Autism Dev Disord 34(2):229–235
Evans DW, Canavera K, Kleinpeter FL, Maccubbin E, Taga K (2005) The fears, phobias, and anxieties of children with autism spectrum disorders and down syndrome: comparison with developmentally and chronologically age matched children. Child Psychiatry Hum Dev 36(1):3–26
Corbett BA, Mendoza S, Abdullah M, Wegelin JA, Levine S (2006) Cortisol circadian rhythms and response to stress in children with autism. Psychoneuroendocrinology 31:59–68
Klimes-Dougan B, Hastings PD, Granger DA, Usher BA, Zahn-Waxler C (2001) Adrenocortical activity in at-risk and normally developing adolescents: individual differences in salivary cortisol basal levels, diurnal variations, and responses to social challenges. Dev Psychopathol 13:695–719
Curin JM, Terzie J, Petkovic ZB, Zekban L, Terzic IM, Susnjara IM (2003) Lower cortisol and higher ACTH levels in individuals with autism. J Autism Dev Disord 33(4):443–447
Nir E, Meir D, Zilber N, Knobler H, Hadjez J, Lerner Y (1995) Brief report: circadian melatonin, thyroid-stimulating hormone, prolactin, and cortisol levels in serum of young adults with autism. J Autism Dev Disord 25(6):641–654
Kirschbaum C, Read GF, Hellhammer D (1992) Assessment of hormones and drugs in saliva in biobehavioral research. Hogrefe and Huber, Seattle
Jansen LMC, Weid CCG, van der Gaag J, van Engeland H (2003) Differentiation between autism and multiple complex developmental disorders in response to psychosocial stress. Neuropsychopharmacology 28:582–590
Strazdins L, Meyerkort S, Brent V, D’Souza RM, Broom DH, Kyd JM (2005) Impact of saliva collection methods on sIgA and cortisol assays and acceptability to participants. J Immunol Methods 307:167–171
Granger DA, Kivlighan KT, Fortunato C, Harmon AG, Hibel LC, Schwartz EB, Whembolua GL (2007) Integration of salivary biomarkers into developmental and behaviorally-oriented research: problems and solutions for collecting specimens. Physiol Behav 92(4):583–590
Gröschl M, Rauh M (2006) Influence of commercial collection devices for saliva on the reliability of salivary steroids analysis. Steroids 71:1097–1100
Shirtcliff EA, Granger DA, Schwartz E, Curran MJ (2001) Use of salivary biomarkers in biobehavioral research: cotton-based sample collections can interfere with salivary immunoassay results. Psychoneuroendocrinology 26(2):165–173
Kozaki T, Hashiguchi N, Kaji Y, Yasukouchi A, Tochihara Y (2009) Effects of saliva collection using cotton swab on cortisol enzyme immunoassay. Eur J Appl Physiol 107(6):743–746
Gallagher P, Leitch MM, Massey AE, McAllister-Williams RH, Young AH (2006) Assessing cortisol and dehydroepiandrosterone (DHEA) in saliva: effects of collection method. J Psychopharmacol 20(5):643–649
Poll EM, Kreitschmann-Andermahr I, Langejuergen Y, Stanzel S, Gilsbach JM, Yagmur E (2007) Saliva collection method affects predictability of serum cortisol. Int J Clin Chem 382(1–2):15–19
Donzella B, Talge NM, Smith TL, Gunnar MR (2008) To spear or not to spear: comparison of saliva collection methods. Dev Psychobiol 50(7):714–717
Kidd S, Midgley P, Lone N, Wallace AM, Nicol M, McIntosh N (2009) A re-investigation of saliva collection procedures that highlights the risk of potential positive interference in cortisol immunoassay. Steroids 74(8):666–668
Hanrahan K, McCarthy AM, Kleiber C, Lutgendorf S, Tsalikian E (2006) Strategies for salivary cortisol collection and analysis in research with children. Appl Nurs Res 19:95–101
Wechsler D (2003) Wechsler intelligence scale for children, 4th edn. The Psychological Corporation, San Antonio
Carrow-Woolfolk E (1999) Comprehensive assessment of spoken language. American Guidance Service, Circle Pines
Rutter M, LeCouteur A, Lord C (2003) Autism diagnostic interview-revised. Western Psychological Services, Los Angeles
Tellegen A, Briggs PF (1967) Old wine in new skins: grouping wechsler subtests into new scales. J Consult Clin Psychol 31:499–506
Mazefsky CA, Oswald DP (2006) The discriminative ability and diagnostic utility of the ADOS-G, ADI-R, and GARS for children in a clinical setting. Autism 10(6):533–549
Stevens J (1996) Applied multivariate statistics for the social sciences, 3rd edn. Lawrence Erlbaum, Mahwah
Cohen J (1988) Statistical power analysis for the behavioral sciences, 2nd edn. Lawrence Erlbaum, Hillsdale
Corbett BA, Mendoza S, Wegelin JA, Carmean V, Levine S (2008) Variable cortisol circadian rhythms in children with autism and anticipatory stress. J Psychiatry Neurosci 33(3):227–234
Corbett BA, Schupp CW, Levine S, Mendoza S (2009) Comparing cortisol, stress, and sensory sensitivity in children with autism. Autism Res 2:39–49
Corbett BA, Schupp CW, Simon D, Ryan N, Mendoza S (2010) Elevated cortisol during play is associated with age and social engagement in children with autism. Mol Autism 1(1):3
Zinke K, Fries E, Kliegel M, Kirschbaum C, Dettenborn L (2010) Children with high-functioning autism show a normal cortisol awakening response (CAR). Psychoneuroendocrinology 35:1578–1582
Viau R, Arsenault-Lapierre G, Fecteau S, Champagne N, Walker CD, Lupien S (2010) Effects of service dogs on salivary cortisol secretion in autistic children. Psychoneuroendocrinology 35(8):1187–1193
Acknowledgments
A portion of the data in this study was collected as part of work supported by Department of Education, Institute of Education Sciences Grant R324A080136. Findings and conclusions are those of the authors and do not necessarily reflect the views of the funding agency.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Putnam, S.K., Lopata, C., Fox, J.D. et al. Comparison of Saliva Collection Methods in Children with High-Functioning Autism Spectrum Disorders: Acceptability and Recovery of Cortisol. Child Psychiatry Hum Dev 43, 560–573 (2012). https://doi.org/10.1007/s10578-012-0284-3
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10578-012-0284-3