Skip to main content
SpringerLink
Log in

Cytogenetic anchoring of radiation hybrid and virtual maps of sheep chromosome X and comparison of X chromosomes in sheep, cattle, and human

  • Published:
Chromosome Research Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

A comprehensive physical map was generated for Ovis aries chromosome X (OARX) based on a cytogenomics approach. DNA probes were prepared from bacterial artificial chromosome (BAC) clones from the CHORI-243 sheep library and were assigned to G-banded metaphase spreads via fluorescence in-situ hybridization (FISH). A total of 22 BACs gave a single hybridization signal to the X chromosome and were assigned out of 32 tested. The positioned BACs contained 16 genes and a microsatellite marker which represent new cytogenetically mapped loci in the sheep genome. The gene and microsatellite loci serve to anchor between the existing radiation hybrid (RH) and virtual sheep genome (VSG) maps to the cytogenetic OARX map, whilst the BACs themselves also serve as anchors between the VSG and the cytogenetic maps. An additional 17 links between the RH and cytogenetic maps are provided by BAC end sequence (BES) derived markers that have also been positioned on the RH map. Comparison of the map orders for the cytogenetic, RH, and virtual maps reveals that the orders for the cytogenetic and RH maps are most similar, with only one locus, represented by BAC CH243-330E18, mapping to relatively different positions. Several discrepancies, including an inverted segment are found when comparing both the cytogenetic and RH maps with the virtual map. These discrepancies highlight the value of using physical mapping methods to inform the process of future in silico map construction. A detailed comparative analysis of sheep, human, and cattle mapping data allowed the construction of a comparative map that confirms and expands the knowledge about evolutionary conservation and break points between the X chromosomes of the three mammalian species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

BAC:

bacterial artificial chromosome

BES:

BAC end sequence

BTA:

Bos taurus

CATS:

comparative anchor tagged sequences

FISH:

fluorescence in-situ hybridization

FITC:

fluorescein isothiocyanate

HSA:

Homo sapiens

ISCNDB:

International System for Chromosome Nomenclature of Domestic Bovids

OAR:

Ovis aries

PI:

propidium iodide

RH:

radiation hybrid

VSG:

virtual sheep genome

References

  • Cockett NE (2006) The sheep genome. Genome Dyn 2:79–85

    Article  PubMed  CAS  Google Scholar 

  • Cribiu EP, Di Berardino D, Di Meo GP et al (2001) International System for Chromosome Nomenclature of Domestic Bovids (ISCNDB 2000). Cytogenet Cell Genet 92:283–299

    Article  PubMed  CAS  Google Scholar 

  • Dalrymple BP, Kirkness EF, Nefedov M, International Sheep Genomics Consortium et al (2007) Using comparative genomics to reorder the human genome sequence into a virtual sheep genome. Genome Biol 8:R152

    Article  PubMed  Google Scholar 

  • Di Meo GP, Perucatti A, Incarnato D et al (2002) Comparative mapping of twenty-eight bovine loci in sheep (Ovis aries, 2n = 54) and river buffalo (Bubalus bubalis, 2n = 50) by FISH. Cytogenet Genome Res 98:262–264

    Article  PubMed  Google Scholar 

  • Di Meo GP, Perucatti A, Gautier M et al (2003) Chromosome localization of the 31 type I Texas bovine markers in sheep and goat chromosomes by comparative FISH-mapping and R-banding. Anim Genet 34:294–296

    Article  PubMed  Google Scholar 

  • Di Meo GP, Perucatti A, Floriot S et al (2005) Chromosome evolution and improved cytogenetic maps of the Y chromosome in cattle, zebu, river buffalo, sheep and goat. Chromosome Res 13:349–355

    Article  PubMed  Google Scholar 

  • Di Meo GP, Perucatti A, Floriot S et al (2007) An advanced sheep (Ovis aries, 2n = 54) cytogenetic map and assignment of 88 new autosomal loci by fluorescence in situ hybridization and R-banding. Anim Genet 38:233–240

    Article  PubMed  Google Scholar 

  • Dobigny G, Yang F (2008) Foreword: Comparative cytogenetics in the genomics era: cytogenomics comes of age. Chromosome Res 16:1–4

    Article  PubMed  CAS  Google Scholar 

  • Fortna A, Kim Y, MacLaren E et al (2004) Lineage-specific gene duplication and loss in human and great ape evolution. PLoS Biol 2:E207

    Article  PubMed  Google Scholar 

  • Goldammer T, Brunner RM, Weikard R, Kuehn C, Wimmers K (2007) Generation of an improved cytogenetic and comparative map of Bos taurus chromosome BTA27. Chromosome Res 15:203–213

    Article  PubMed  CAS  Google Scholar 

  • Goldammer T, Brunner RM, Rebl A et al. (2009) A high-resolution radiation hybrid map of sheep chromosome X and comparison with human and cattle. Cytogenet Genome Res 124: (DOI:10.1159/000207520, in press).

  • Hecht J, Kuhl H, Haas SA et al (2006) Gene identification and analysis of transcripts differentially regulated in fracture healing by EST sequencing in the domestic sheep. BMC Genomics 7:172

    Article  PubMed  Google Scholar 

  • Iannuzzi L, Di Meo GP, Perucatti A et al (2000) Comparative FISH-mapping of bovid X chromosomes reveals homologies and divercences between the subfamilies Bovinae and Caprinae. Cytogenet Cell Genet 89:171–176

    Article  PubMed  CAS  Google Scholar 

  • Lyons LA, Laughlin TF, Copeland NG et al (1997) Comparative anchor tagged sequences (CATS) for integrative mapping of mammalian genomes. Nature Genet 15:47–56

    Article  PubMed  CAS  Google Scholar 

  • Maddox JF, Davies KP, Crawford AM et al (2001) An enhanced linkage map of the sheep genome comprising more than 1000 loci. Genome Res 11:1275–1289

    Article  PubMed  CAS  Google Scholar 

  • Mandon-Pépin B, Oustry-Vaiman A, Vigier B, Piumi F, Cribiu E, Cotinot C (2003) Expression profiles and chromosomal localization of genes controlling meiosis and follicular development in the sheep ovary. Biol Reprod 68:985–995

    Article  PubMed  Google Scholar 

  • Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938

    Article  PubMed  CAS  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386

    PubMed  CAS  Google Scholar 

  • Seabright M (1971) A rapid banding technique for human chromosomes. Lancet 2:971

    Article  PubMed  CAS  Google Scholar 

  • Tetens J, Goldammer T, Maddox JF, Cockett NE, Leeb T, Drögemüller C (2007) A radiation hybrid map of sheep chromosome 23 based on ovine BAC-end sequences. Anim Genet 38:132–140

    Article  PubMed  CAS  Google Scholar 

  • Wain HM, Bruford EA, Lovering RC, Lush MJ, Wright MW, Povey S (2002) Guidelines for human gene nomenclature. Genomics 79:464–470

    Article  PubMed  CAS  Google Scholar 

  • Watanabe H, Fujiyama A, Hattori M et al (2004) DNA sequence and comparative analysis of chimpanzee chromosome 22. Nature 429:382–388

    Article  PubMed  CAS  Google Scholar 

  • Wu CH, Nomura K, Goldammer T, Hadfield T, Womack JE, Cockett NE (2007) An ovine whole-genome radiation hybrid panel used to construct an RH map of ovine chromosome 9. Anim Genet 38:534–536

    Article  PubMed  CAS  Google Scholar 

  • Wu CH, Hadfield T, Dalrymple BP et al (2009) Using the ovine radiation hybrid map to enhance comparisons of the ovine, bovine and human genomes. Plant & Animal Genomes XVII Conference, San Diego, CA, USA, p 496

    Google Scholar 

  • Yunis JJ, Prakash O (1982) The origin of man: a chromosomal pictorial legacy. Science 215:1525–1530

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We gratefully thank Brigitte Schöpl, Ingrid Hennings, and Janine Vogt for excellent technical assistance in the mapping procedure. We thank Roland Riebe from FLI-Insel Riems for providing the embryonic sheep fibroblast cell line (SE-R). DFG-Grant GO-896/6-3 from the German Research Foundation supported the comparative mapping study.

Author information

Authors and Affiliations

  1. Forschungsbereich Molekularbiologie, Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Wilhelm-Stahl-Allee 2, D-18196, Dummerstorf, Germany

    Tom Goldammer, Ronald M. Brunner & Alexander Rebl

  2. Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT, USA

    Chun Hua Wu, Tracy Hadfield & Noelle E. Cockett

  3. Department of Animal Science, Tokyo University of Agriculture, Kanagawa, Japan

    Ko Nomura

  4. Department of Veterinary Science, University of Melbourne, Victoria, Australia

    Jill F. Maddox

Authors
  1. Tom Goldammer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ronald M. Brunner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alexander Rebl
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chun Hua Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ko Nomura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tracy Hadfield
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jill F. Maddox
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Noelle E. Cockett
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tom Goldammer.

Additional information

Responsible Editor: Herbert Macgregor.

Accession numbers: Sequence data from this article have been deposited with the GenBank Data Library under Accession Nos. FJ853178–FJ853188 and FJ868495–FJ868499.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goldammer, T., Brunner, R.M., Rebl, A. et al. Cytogenetic anchoring of radiation hybrid and virtual maps of sheep chromosome X and comparison of X chromosomes in sheep, cattle, and human. Chromosome Res 17, 497–506 (2009). https://doi.org/10.1007/s10577-009-9047-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10577-009-9047-9

Keywords

Search

Navigation