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Chromosome evolution in the subtribe Bovina (Mammalia, Bovidae): The karyotype of the Cambodian banteng (Bos javanicus birmanicus) suggests that Robertsonian translocations are related to interspecific hybridization

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Abstract

Three subspecies of banteng (Bos javanicus) have been described: B. j. javanicus in Java, B. j. lowi in Borneo, and B. j. birmanicus in Cambodia, Lao PDR, Myanmar, Thailand and Vietnam. In this paper we provide the first description of the karyotype of the Cambodian banteng. The chromosomal complement of B. j. birmanicus differs from that of B. j. javanicus, which was previously found to be similar to that of cattle, Bos taurus (2n = 60). The Cambodian banteng karyotype has a diploid number of 2n = 56 (FN = 62) and the karyotype consists of 26 pairs of acrocentric chromosomes and two pairs of submetacentric chromosomes. Comparisons with other species of the subtribe Bovina show that the two pairs of bi-armed chromosomes resulted from two centric fusions involving the equivalent of cattle chromosomes 1 and 29, and 2 and 28, respectively. Cross-species fluorescence in-situ hybridization (FISH) with B. taurus whole chromosome paints and satellite DNA I probes was used to identify the chromosomes involved in the translocations, and their orientation. We suggest that Robertsonian translocations (1;29) and (2;28) have been fixed in the common ancestor of Cambodian banteng as a consequence of hybridization with the kouprey (Bos sauveli) during the Pleistocene epoch.

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Abbreviations

BJAb:

Bos javanicus birmanicus

BJAj:

Bos javanicus javanicus

BrdU:

5-bromo-2-deoxyuridine

BTA:

Bos taurus

DMEM:

Dulbecco’s Modified Eagle Medium

dUTP:

deoxyuridine triphosphate

FISH:

fluorescence in-situ hybridization

FN:

fundamental number

Mya:

million years ago

PCR:

polymerase chain reaction

Rob:

Robertsonian

SSC:

saline-sodium citrate

TN:

Tris-NaCl

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Acknowledgements

Biopsies were done in the Phnom Tamao zoo by Norin Chai thanks to the help of Nick Marx, Charly Pignon, Yannick Roman, Roland Simon, and all the keepers of the Rescue Center. We also acknowledge Johannes A. Lenstra for bibliographic help and Aurora Ruiz-Herrera and Maud Bonato for helpful comments. V.R.I. was funded through a research grant MZE 0002716201. A.R. and A.H. were supported by funds from the MNHN, CNRS, and PPF ‘État et structure phylogénétique de la biodiversité actuelle et fossile’. A.R. gratefully acknowledges the National Research Foundation of South Africa (GUN 65079).

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Authors and Affiliations

  1. UMR 5202 – Origine, Structure et Evolution de la Biodiversité Département Systématique et Evolution, Muséum National d’Histoire Naturelle, Case postale No. 51, 55, rue Buffon, 75005, Paris, France

    Anne Ropiquet, Michèle Gerbault-Seureau & Alexandre Hassanin

  2. Department of Botany & Zoology – Evolutionary Genomics Group, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa

    Jane L. Deuve

  3. Department of Biology, University of Texas at Arlington, PO Box 19498, Arlington, TX, 76019, USA

    Clément Gilbert

  4. Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic

    Eva Pagacova & Jiri Rubes

  5. USM 0801 Ménagerie du Jardin des Plantes, Département des Jardins Botaniques et Zoologiques, Muséum National d’Histoire Naturelle, 57 rue Cuvier, 75005, Paris, France

    Norin Chai

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  1. Anne Ropiquet
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  2. Michèle Gerbault-Seureau
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Correspondence to Anne Ropiquet or Alexandre Hassanin.

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Ropiquet, A., Gerbault-Seureau, M., Deuve, J.L. et al. Chromosome evolution in the subtribe Bovina (Mammalia, Bovidae): The karyotype of the Cambodian banteng (Bos javanicus birmanicus) suggests that Robertsonian translocations are related to interspecific hybridization. Chromosome Res 16, 1107–1118 (2008). https://doi.org/10.1007/s10577-008-1262-2

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