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Dynamic Change of Prohibitin2 Expression in Rat Sciatic Nerve After Crush

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Abstract

As a novel cell cycle inhibitor, PHB2 controls the G1/S transition in cycling cells in a complex manner. Its aberrant expression is closely related to cell carcinogenesis. While its expression and role in peripheral nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve crush (SNC) model in adult rats to examine the dynamic changes of PHB2. Temporally, PHB2 expression was sharply decreased after sciatic nerve crush and reached a valley at day 5. Spatially, PHB2 was widely expressed in the normal sciatic nerve including axons and Schwann cells. While after injury, PHB2 expression decreased predominantly in Schwann cells. The alteration was due to the decreased expression of PHB2 in Schwann cells after SNC. PHB2 expression correlated closely with Schwann cells proliferation in sciatic nerve post injury. Furthermore, PHB2 largely localized with GAP43 in axons in the crushed segment. Collectively, we suggested that PHB2 participated in the pathological process response to sciatic nerve injury and may be associated with Schwann cells proliferation and axons regeneration.

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Abbreviations

PHB2:

Prohibitin2

GAP43:

Growth-associated protein 43

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

PBS:

Phosphate-buffered saline

PNS:

Peripheral nervous system

PCNA:

Proliferating cell nuclear antigen

SCs:

Schwann cells

SNC:

Sciatic nerve crush

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 81171140, 30300099, 30770488 and 30870320); Natural Science Foundation of Jiangsu province (Nos. BK2009161, BK2009156, BK2009157); Natural science Foundation of Jiangsu Colleges and Universities Grant (09KJD310005).

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Hospital of Nantong University, 20 Xi-Si Road, Nantong, 226001, Jiangsu, People’s Republic of China

    Long Long, Hao Wu, Hai Wen, Tao Ding & Youhua Wang

  2. Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Yan Huang

  3. Department of Neurosurgery, Nanjing Medical University, Nanjing, 210029, Jiangsu, People’s Republic of China

    Wenkang Luan

  4. Department of Medical Imaging, Clinical School of Medical College, Nanjing University, Nanjing, 210009, Jiangsu, People’s Republic of China

    Qi Zhang

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  1. Long Long
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  2. Yan Huang
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  3. Hao Wu
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Correspondence to Youhua Wang.

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Long, L., Huang, Y., Wu, H. et al. Dynamic Change of Prohibitin2 Expression in Rat Sciatic Nerve After Crush. Cell Mol Neurobiol 33, 689–698 (2013). https://doi.org/10.1007/s10571-013-9935-6

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