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The Protective Mechanism for the Blood–Brain Barrier Induced by Aminoguanidine in Surgical Brain Injury in Rats

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Abstract

This study was performed to investigate the mechanism of blood–brain barrier (BBB) permeability change, which was induced by aminoguanidine (AG) after surgical brain injury (SBI) in rats. Compared to control group, AG (150 mg/kg, i.p.) significantly reduced Evans blue extravasation into brain tissue at 24 h after surgical resection, it also induced a 32% decrease of malondialdehyde (MDA) values and a 1.1-fold increase of the glutathione (GSH) levels at 12 h after injury. The expression of inducible nitric oxide synthase (iNOS) reached the peak value at 24 h after SBI, which was significantly attenuated after AG treatment. In addition, ZO-1 protein was up-regulated by AG (150 mg/kg) treatment at 24 h after SBI. Our results indicated that AG could protect the BBB after SBI, which could be correlated with antioxidative property, the down-regulation of iNOS and up-regulation of tight junction protein expression.

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Acknowledgments

This work was supported by the beginning Science Foundation of the General Hospital of Shenyang military region, No 09Y-Q11, Doctor-beginning Science Foundation of Liaoning Province, No.20101109, and special fund for scientific research of doctor-degree subject for the new teacher in colleges and universities, No.20102134120007.

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Authors and Affiliations

  1. Department of Anatomy, College Basic of Medical Sciences, China Medical University, Shenyang, 110001, Liaoning Province, People’s Republic of China

    Di Fan & Xiao-jie Tong

  2. Department of Neurosurgery, The General Hospital of Shenyang Military Region, Shenyang, 110016, Liaoning Province, People’s Republic of China

    Di Fan, Tao Tang, Zai-hua Xu & Zhen-quan Song

  3. Department of Physiology, Life Science and Biology Pharmacopedia Institution, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning Province, People’s Republic of China

    Yan-ting Gu

  4. Department of Prosthodontics, Shenyang Stomatology Hospital, Shenyang, 110001, Liaoning Province, People’s Republic of China

    Hui Lv

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  1. Di Fan
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  2. Yan-ting Gu
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  3. Hui Lv
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  4. Tao Tang
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  5. Zai-hua Xu
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  7. Xiao-jie Tong
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Correspondence to Xiao-jie Tong.

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Fan, D., Gu, Yt., Lv, H. et al. The Protective Mechanism for the Blood–Brain Barrier Induced by Aminoguanidine in Surgical Brain Injury in Rats. Cell Mol Neurobiol 31, 1213–1219 (2011). https://doi.org/10.1007/s10571-011-9723-0

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  • DOI: https://doi.org/10.1007/s10571-011-9723-0

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