Abstract
(1) A new human glioblastoma multiforme (GBM) cell line, WJ1, was established from the tissue derived from a 29-year-old patient diagnosed with a grade IV GBM. (2) The WJ1 cell line has been subcultured for more than 80 passages in standard culture media without feeder layer or collagen coatings. (3) GBM cells grow in vitro with distinct morphological appearance. Ultrastructural examination revealed large irregular nuclei and pseudo-inclusion bodies in nuclei. The cytoplasm contained numerous immature organelles and a few glia filaments. Growth kinetic studies demonstrated an approximate population doubling time of 60 h and a colony forming efficiency of 4.04%. The karyotype of the cells was hyperdiploid, with a large subpopulation of polyploid cells. Drug sensitivities of DDP, VP-16, tanshinone IIA of this cell line were assayed. They showed a dose- and time-dependent growth inhibition effect on the cells. (4) Orthotopic transplantation of GBM cells into athymic nude mice induced the formation of solid tumor masses about 6 weeks. The cells obtained from mouse tumor masses when cultivated in vitro had the same morphology and ultrastructure as those of the initial cultures. (5) This cell line may provide a useful model in vitro and in vivo in the cellular and molecular studies as well as in testing novel therapies for human glioblastoma multiforme.
This is a preview of subscription content, log in via an institution to check access.
Reference
Bacciocchi G, Gibelli N, Zibera C, Pedrazolli P, Bergamaschi G, Polver PDP, Danova M, Mazzini G, Palomba L, Tupler R, Cuna GRD (1992) Establishment and characterization of two cell lines derived from human glioblastoma multiforme. Anticancer Res 12:853–862
Bakir A, Gezen F, Yildiz O, Ayhan A, Kahraman S, Kruse CA, Varella-Garcia M, Yildiz F, Kubar A (1998) Establishment and characterization of a human glioblastoma multiforme cell line. Cancer Genet Cytogenet 103:46–51
Begemann M, Fuller GN, Holland EC (2002) Genetic modeling of glioma formation in mice. Brain Pathol 12:117–132
Davis FG, Freels S, Grutsch J, Barlas S, Brem S (1998) Survival rates in patients with primary malignant brain tumors stratified by patient age and tumor histological type: An analysis based on Surveillance, Epidemiology, and End Results (SEER) data, 1973-1991. J Neurosurg 88:1–10
Di Tomaso E, Pang JCS, Ng HK, Lam PYP, Tian XX, Suen KW, Hui ABY, Hjelm N M (2000) Establishment and characterization of a human cell line from paedriatic cerebellar glioblastoma multiform. Neuropathol Appl Neurobiol 26:22–30
Fomchenko EI, Holland EC (2006) Mouse models of brain tumors and their applications in preclinical trials. Clin Cancer Res 12:5288–5297
Grippo MC, Penteado PF, Carelli EF, Cruz-Holfing MA, Verinaud L (2001) Establishment and partial characterization of a continuous human malignant glioma cell line: NG97. Cell Mol Neurobiol 21:421–428
Gutmann DH, Baker SJ, Giovannini M, Garbow J, Weiss W (2003) Mouse models of human cancer consortium symposium on nervous system tumors. Cancer Res 63:3001–3004
Holland EC (2001) Gliomagenesis: genetic alterations and mouse models. Nat Rev Genet 2:120–129
Horiguchi H, Matsui M, Ahtsubo R, Ogata T, Kaneko M, Fujiwara M, Kamma H (1998) Establishment and characterization of a typical primitive neuroectodermal tumor cell line. In vitro Cell Dev Biol Anim 34:439–442
Jost SC, Wanebo JE, Song SK, Chicoine MR, Rich KM, Woolsey TA, Lewis JS, Mach RH, Xu J, Garbow JR (2007) In vivo imaging in a murine model of glioblastoma. Neurosurgery 60:360–371
Lyustikman Y, Lassman AB (2006) Glioma oncogenesis and animal models of glioma formation. Hematol Oncol Clin North Am 20:1193–1214
Maher EA, Furnari FB, Bachoo RM, Rowitch DH, Louis DN, Cavenee WK, De Pinho RA (2001) Malignant glioma:genetics and biology of a grave matter. Genes Dev 15:1311–1333
Selvakumaran M, Pisarcik DA, Bao R, Yeung AT, Hamilton TC (2003) Enhanced cisplatin cytotoxicity by disturbing the nucleotide excision repair pathway in ovarian cancer cell lines. Cancer Res 63:1311–1316
Singh SK, Clarke ID, Terasaki M, Bone VE, Hawkins CH, Squire J, Dirks PB (2003). Identification of a cancer stem cell in human brain tumors. Cancer Res 63:5821–5828
Singh SK, Hawkins C, Clarke ID, Squire JA, TakuichiroHide BA, Henkelman RM, Cusimano MD, Dirks PB (2004) Identification of human brain tumors initiating cells. Nature 432:396–401
Smilowitz HM, Weissenberger J, Weis J, Brown JD, O’Neill RJ, Laissue JA (2007) Orthotopic transplantation of v-src-expressing glioma cell lines into immunocompetent mice: establishment of a new transplantable in vivo model for malignant glioma. J Neurosurg 106:652–659
Surawicz TS, Davis F, Freels S, Laws ER Jr, Menck HR (1998) Brain tumor survival: results from the National Cancer Data Base. J Neurooncol 40:151–160
Voskoglou-Nomikos T, Pater JL, Seymour L (2003) Clinical predictive value of the in vitro cell line, human xenograft, and mouse allograft preclinical cancer models. Clin Cancer Res 9:4227–4239
Wang J, Wang XJ, Jiang S, Yuan SL, Lin P, Zhang J, Lu YR, Wang Q, Xiong ZJ, Wu YY, Ren JJ, Yong HL (2007) rowth inhibition and induction of apoptosis and differentiation of tanshinone IIA in human glioma cells. J Neuro-oncol 82:11–21
Acknowledgments
This study was supported by National Natural Science Foundation of China; Grant Number: 30471779.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, J., Wang, X., Jiang, S. et al. Establishment of a New Human Glioblastoma Multiforme Cell Line (WJ1) and Its Partial Characterization. Cell Mol Neurobiol 27, 831–843 (2007). https://doi.org/10.1007/s10571-007-9188-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10571-007-9188-3