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Ultrastructure of parenchyma cell wall in bamboo (Phyllostachys edulis) culms

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Abstract

Parenchyma cell wall structure plays a crucial role in the growth and the mechanical properties of bamboo plants, with the secondary cell wall providing strength and rigidity. However, little is known about the ultrastructure of the parenchyma cell wall. The aim of this study was to characterize the anatomical structure of the parenchyma cell wall and determine how it contributes to great mechanical superiority of bamboo culm. We investigated the ultrastructure of the parenchyma cell wall using transmission electron microscopy and field-emission environmental scanning electron microscopy. The key results show that the secondary cell walls of ground and vascular parenchyma cells exhibited tight-loose (light-dark) alternating layers. The pit membrane of the ground parenchyma cells contained numerous pores, and that of vascular parenchyma cells contained some plasmodesmata. Secondary cell walls of most bamboo parenchyma cells contained seven sub-layers, with a maximum of eleven sub-layers of ground parenchyma cells and nine sub-layers of vascular parenchyma cells. The average thickness of ground parenchyma cell wall sub-layers was higher than that in vascular parenchyma cells. The pit membrane thickness of ground parenchyma cells was also higher than that of vascular parenchyma cells, but the diameter of the ground parenchyma cells was smaller than that of the vascular parenchyma cells. The extremely high flexibility of moso bamboo stem could be the consequence of the presence of secondary cell walls in parenchyma cells, and its ultrastructure.

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References

  • An X (2014) Microfibril orientations and ultrastructures of fibers wall from Moso Bamboo. Ph.D. dissertation, Chinese Academy of Forestry, Beijing, China

  • Casdorff K, Keplinger T, Rüggeber M, Burgert I (2018) A close-up view of the wood cell wall ultrastructure and its mechanics at different cutting angles by atomic force microscopy. Planta 247:1123–1132

    Article  CAS  Google Scholar 

  • Chen H (2014) Study on the structural characteristics of bamboo cell wall. Ph.D. dissertation, Chinese Academy of Forestry, Beijing, China

  • Chen M, Fei B (2018) In-situ Obeservation on the morphological behavior of bamboo under flexural stress with respect to its fiber-foam composite structure. Bioresources 13:5472–5478

    CAS  Google Scholar 

  • Chen M, Ye L, Li H, Wang G, Chen Q, Fang C, Dai C, Fei B (2020) Flexural strength and ductility of moso bamboo. Constr Build Mater 246:118418

    Article  Google Scholar 

  • Choat B, Cobb AR, Jansen S (2008) Structure and function of bordered pits: new discoveries and impacts on whole-plant hydraulic function. New Phytol 177:608–625

    Article  Google Scholar 

  • Cosgrove DJ, Jarvis MC (2012) Comparative structure and biomechanics of plant primary and secondary cell walls. Front Plant Sci 3:204

    Article  Google Scholar 

  • Donaldson L (2008) Review- Microfibril angle: measurement, variation and relationship. IAWA Journal 29:345–386

    Article  Google Scholar 

  • Evert RF (2006) Esau’s plant anatomy: meristems, cells, and tissues of the plant body: their structure, function, and development. 3rd Edn. Wiley, Hoboken

    Book  Google Scholar 

  • Fei B, Liu R, Liu X, Chen X, Zhang S (2019) A review of structure and characterization methods of bamboo pits. J For Eng 4:13–18

    Google Scholar 

  • Fromm J (2013) Cellular aspects of wood formation. Springer, Heidelberg

    Book  Google Scholar 

  • Gan X, Ding Y (2006) Investigation on the variation of fiber wall in Phyllostachys edulis culms. For Res 19:457

    Google Scholar 

  • Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315:804–807

    Article  CAS  Google Scholar 

  • Hu K, Huang Y, Fei B, Yao C, Zhao C (2017) Investigation of the multilayered structure and microfibril angle of different types of bamboo cell walls at the micro/nano level using a LC-PolScope imaging. Cellulose. DOI https://doi.org/10.1007/s10570-017-1447-y

  • Jansen S, Lamy JB, Burlett R, Cochard H, Gasson P, Delzon S (2012) Plasmodesmatal pores in the torus of bordered pit membranes affect cavitation resistance of conifer xylem. Plant Cell Environ 35:1109–1120

    Article  CAS  Google Scholar 

  • Jiang ZH (2007) Bamboo and Rattan in the world. China Forestry Publishing House, Beijing

    Google Scholar 

  • Kenneth K (2010) Plant cell walls. Plant Physiol 154:483–486

    Article  Google Scholar 

  • Li ZL (1983) The plant anatomy. Senior Education Press, Beijing

    Google Scholar 

  • Lian C, Liu R, Cheng X, Zhang S, Luo J, Yang S, Liu X, Fei B (2019a) Characterization of the pits in parenchyma cells of the moso bamboo [Phyllostachys edulis (Carr.) J. Houz.] culm. Holzforschung 73:629–636

    Article  CAS  Google Scholar 

  • Lian C, Zhang S, Liu X, Luo J, Yang F, Liu R, Fei B (2019b) Uncovering the ultrastructure of ramiform pits in the parenchyma cells of bamboo (phyllostachys edulis (Carr.) J. Houz.). Holzforschung, https://doi.org/10.1515/hf-2019-0166

  • Liese W (1998) The anatomy of bamboo culms. International Network for Bamboo and Rattan, Beijing

    Google Scholar 

  • Parameswaran N, Liese W (1976) On the fine structure of bamboo fibres. Wood Sci Technol 10:231–246

    CAS  Google Scholar 

  • Preston R, Singh K (1950) The fine structure of bamboo fibres I. Optical properties and X-ray data. J Exp Bot 1:214–226

    Article  Google Scholar 

  • Ren D, Wang H, Yu Z, Wang H, Yu Y (2015) Mechanical imaging of bamboo fiber cell walls and their composites by means of peakforce quantitative nanomechanics (PQNM) technique. Holzforschung 69:975–984

    Article  CAS  Google Scholar 

  • Rüggeberg M, Saxe F, Metzger TH, Sundberg B, Fratzl P, Burgert I (2013) Enhanced cellulose orientation analysis in complex model plant tissues. J Struct Biol 183:419–428

    Article  Google Scholar 

  • Schulte PJ, Hacke UG, Schoonmaker AL (2015) Pit membrane structure is highly variable and accounts for a major resistance to water flow through tracheid pits in stems and roots of two boreal conifer species. New Phytol 208:102–113

    Article  Google Scholar 

  • Singh A, Daniel G, Nilsson T (2002) Ultrastructure of S2 layer in relation to lignin distribution in Pinus radiata tracheids. J Wood Sci 48:95–98

    Article  CAS  Google Scholar 

  • Underwood W (2012) The plant cell wall: a dynamic barrier against pathogen invasion. Front Plant Sci 3:85

    Article  CAS  Google Scholar 

  • Wang X, Keplinger T, Gierlinger N, Burgert I (2014) Plant material features responsible for bamboo\“s excellent mechanical performance: a comparison of tensile properties of bamboo and spruce at the tissue, fibre and cell wall levels. Ann Bot 114:1627–1635

    Article  CAS  Google Scholar 

  • Yu Y, Wang H, Lu F, Tian G, Lin J (2014) Bamboo fibers for composite applications: a mechanical and morphological investigation. J Mater Sci 49:2559–2566

    Article  CAS  Google Scholar 

  • Zhang Y, Klepsch M, Jansen S (2017) Bordered pits in xylem of vesselless angiosperms and their possible misinterpretation as perforation plates. Plant Cell Environ 40:2133–2146

    Article  CAS  Google Scholar 

  • Zhou X, Ding D, Ma J, Ji Z, Zhang X, Xu F (2015) Ultrastructure and Topochemistry of Plant Cell Wall by Transmission Electron Microscopy. https://doi.org/10.5772/60752

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Acknowledgments

The authors acknowledge the financial support from the National Natural Science Foundation (Grant No. 31770599).

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Authors and Affiliations

  1. Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, Department of Bio-materials, International Center for Bamboo and Rattan, Beijing, 100102, China

    Caiping Lian, Rong Liu, Shuqin Zhang, Jing Yuan, Junji Luo & Benhua Fei

  2. School of Materials Science & Engineering, Beijing Institute of Fashion Technology, Beijing, 100029, China

    Feng Yang

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  1. Caiping Lian
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  2. Rong Liu
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  3. Shuqin Zhang
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  4. Jing Yuan
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  5. Junji Luo
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  6. Feng Yang
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  7. Benhua Fei
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Correspondence to Benhua Fei.

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Lian, C., Liu, R., Zhang, S. et al. Ultrastructure of parenchyma cell wall in bamboo (Phyllostachys edulis) culms. Cellulose 27, 7321–7329 (2020). https://doi.org/10.1007/s10570-020-03265-9

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  • DOI: https://doi.org/10.1007/s10570-020-03265-9

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