Skip to main content

Advertisement

SpringerLink
Log in

Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties

  • Communication
  • Published:
Cellulose Aims and scope Submit manuscript

Abstract

This study was conducted to develop nanocomposite films of bacterial cellulose (BC) and montmorillonite (MMT) with potent antibacterial activity and potential therapeutic value in wound healing and tissue regeneration. Different composites were prepared through impregnation of BC sheets with 2 and 4 % suspensions of MMT, Na-MMT, Ca-MMT and Cu-MMT. These modified MMTs were prepared through cation exchange strategy. The antibacterial activities of the composites were then assessed against Escherichia coli and Staphylococcus aureus through the disc diffusion assay and colony forming unit (CFU) count methods. BC-Cu-MMT composites prepared with 2 and 4 % MMT displayed clear zones of inhibition against E. coli (20 and 22 mm, respectively) and S. aureus (19 and 20.5 mm, respectively). The untainted BC, BC-MMT, BC-Na-MMT and BC-Ca-MMT did not show clear inhibitory zones against the tested organisms. The reduction in CFU observed following treatment with BC-MMTs (BC-MMT, BC-Na-MMT, BC-Ca-MMT and BC-Cu-MMT) prepared using 2 % MMTs suspension was 7.39, 14.8, 19.2 and 77.9 % for E. coli and 6.8, 13.7, 17.4 and 74.1 %, for S. aureus, respectively. When treated with BC-MMT, BC-Na-MMT, BC-Ca-MMT and BC-Cu-MMT prepared with 4 % MMTs suspension, the reduction in CFU increased to 10.58, 18.37, 24.62 and 85.01 % for E. coli and 9.44, 15.73, 20.40 and 79.79 % for S. aureus, respectively. The outcome of this study will facilitate the development of BC sheets as wound dressings and regeneration materials with antibacterial properties for therapeutic applications without any side effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Carretero MI (2002) Clay minerals and their beneficial effects upon human health. A review. Appl Clay Sci 21:155–163

    Article  CAS  Google Scholar 

  • Ciechanska D (2004) Multifunctional bacterial cellulose/chitosan composite materials for medical applications. Fibres Text East Eur 12:69–72

    CAS  Google Scholar 

  • Czaja W, Krystynowicz A, Bielecki S, Brown RM Jr (2006) Microbial cellulose—the natural power to heal wounds. Biomaterials 27:145–151

    Article  CAS  Google Scholar 

  • Dahman Y (2009) Nanostructured biomaterials and biocomposites from bacterial cellulose nanofibers. J Nanosci Nanotechnol 9:5105–5122

    Article  CAS  Google Scholar 

  • Emami-Razavi SH, Esmaeili N, Forouzannia SK, Amanpour S, Rabbani S, Alizadeh AM, Mohaghegh MA (2006) Effect of bentonite on skin wound healing: experimental study in the rat model. Acta Medica Iranica 44:235–240

    Google Scholar 

  • Emmerich K, Wolters F, Kahr G, Lagaly G (2009) Clay profiling: the classification of montmorillonites. Clay Clay Miner 57:104–114

    Article  CAS  Google Scholar 

  • Ha JH, Park JK (2012) Improvement of bacterial cellulose production in Acetobacter xylinum using byproduct produced by Gluconacetobacter hansenii. Korean J Chem Eng 29:563–566

    Article  CAS  Google Scholar 

  • Haydel SE, Remenih CM, Williams LB (2008) Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens. J Antimicrob Chemother 61:353–361

    Article  CAS  Google Scholar 

  • Hu CH, Xia MS (2006) Adsorption and antimicrobial effect of copper-exchanged montmorillonite on Escherichia coli K 88. Appl Clay Sci 31:180–184

    Article  CAS  Google Scholar 

  • Hu CH, Xu ZR, Xia MS (2005) Antibacterial effect of Cu2+-exchanged montmorillonite on Aeromonas hydrophila and discussion on its mechanism. Vet Microbiol 109:83–88

    Article  CAS  Google Scholar 

  • Hu WL, Chen S, Li X, Shi S, Shen W, Zhang X, Wang H (2009) In situ synthesis of silver chloride nanoparticles into bacterial cellulose membranes. Mater Sci Eng C Bio S 29:1216–1219

    Article  CAS  Google Scholar 

  • Kheirabadi BS, Mace JE, Terrazas IB, Fedyk CG, Estep JS, Dubick MA, Blackbourne LH (2010a) Safety evaluation of new hemostatic agents, smectite granules, and kaolin-coated gauze in a vascular injury wound model in swine. J Trauma 68:269–278

    Article  CAS  Google Scholar 

  • Kheirabadi BS, Mace JE, Terrazas IB, Fedyk CG, Valdez KK, MacPhee MJ, Beall D, Estep JS, Dubick MA, Blackbourne LH (2010b) Clot-inducing minerals versus plasma protein dressing for topical treatment of external bleeding in the presence of coagulopathy. J Trauma 69:1062–1072

    Article  CAS  Google Scholar 

  • Lee YH, Kuo TF, Chen BY, Feng YK, Wen YR, Lin WC, Lin FH (2005) Toxicity assessment of montmorillonite as a drug carrier for pharmaceutical applications: yeast and rats model. Biomed Eng Appl Basis C 17:72–78

    Article  Google Scholar 

  • Li HX, Kim SJ, Lee YW, Kee CD, Oh IK (2011) Determination of the stoichiometry and critical oxygen tension in the production culture of bacterial cellulose using saccharified food wastes. Korean J Chem Eng 28:2306–2311

    Article  CAS  Google Scholar 

  • Liu H, Chaudhary D, Yusa SI, Tadé MO (2011) Glycerol/starch/Na(+)-montmorillonite nanocomposites: a XRD, FTIR, DSC and 1H NMR study. Carbohydr Polym 83:1591–1597

    Article  CAS  Google Scholar 

  • Maneerung T, Tokura S, Rujiravanit R (2008) Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohydr Polym 72:43–51

    Article  CAS  Google Scholar 

  • Meftahi A, Khajavi R, Rashidi A, Sattari M, Yazdanshenas ME, Torabi M (2010) The effects of cotton gauze coating with microbial cellulose. Cellulose 17:199–204

    Article  CAS  Google Scholar 

  • Meng N, Zhou NL, Zhang SQ, Shen J (2009) Synthesis and antimicrobial activities of polymer/montmorillonite-chlorhexidine acetate nanocomposite films. Appl Clay Sci 42:667–670

    Article  CAS  Google Scholar 

  • Rueda L, Garcia I, Palomares T, Alonso-Varona A, Mondragon I, Corcuera M, Eceiza A (2011) The role of reactive silicates on the structure/property relationships and cell response evaluation in polyurethane nanocomposites. J Biomed Mater Res A 97:480–489

    Google Scholar 

  • Trovatti E, Fernandes SCM, Rubatat L, Freire CSR, Silvestre AJD, Neto CP (2012) Sustainable nanocomposite films based on bacterial cellulose and pullulan. Cellulose 19:729–737

    Article  CAS  Google Scholar 

  • Ul-Islam M, Shah N, Ha JH, Park JK (2011) Effect of chitosan penetration on physico-chemical and mechanical properties of bacterial cellulose. Korean J Chem Eng 28:1736–1743

    Article  CAS  Google Scholar 

  • Ul-Islam M, Khattak WA, Kang M, Kim SM, Khan T, Park JK (2012a) Effect of post-synthetic processing conditions on structural variations and applications of bacterial cellulose. Cellulose. doi:10.1007/s10570-012-9799-9

    Google Scholar 

  • Ul-Islam M, Khan T, Park JK (2012b) Nanoreinforced bacterial cellulose-montmorillonite composites for biomedical applications. Carbohydr Polym 89:1189–1197

    Article  CAS  Google Scholar 

  • Vaiana CA, Leonard MK, Drummy LF, Singh KM, Bubulya A, Vaia RA, Naik RR, Kadakia MP (2011) Epidermal growth factor: layered silicate nanocomposites for tissue regeneration. Biomacromolecules 12:3139–3146

    Article  CAS  Google Scholar 

  • Xia MS, Hu CH, Xu ZR, Ye Y, Zhou YH, Xiong L (2004) Effects of copper-bearing montmorillonite (Cu-MMT) on Escherichia coli and diarrhea on weanling pigs. Asian Aust J Anim Sci 17:1712–1716

    CAS  Google Scholar 

  • Xia MS, Hu CH, Xu Y, Zhao WY (2007) Adsorption of Aeromonas hydrophila by copper-bearing Montmorilliniite clays. J Inorg Mater 22:652–656

    CAS  Google Scholar 

  • Zhang W, Zhang Y, Ji J, Yan Q, Huang A, Chu PK (2007) Antimicrobial polyethylene with controlled copper release. J Biomed Mater Res A 83:838–844

    Google Scholar 

Download references

Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2010-0012672).

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Kyungpook National University, Daegu, 702-701, Korea

    Mazhar Ul-Islam, Taous Khan, Waleed Ahmad Khattak & Joong Kon Park

  2. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan

    Taous Khan

Authors
  1. Mazhar Ul-Islam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Taous Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Waleed Ahmad Khattak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Joong Kon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joong Kon Park.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ul-Islam, M., Khan, T., Khattak, W.A. et al. Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties. Cellulose 20, 589–596 (2013). https://doi.org/10.1007/s10570-012-9849-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10570-012-9849-3

Keywords

Search

Navigation