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Characterizing wood polymers in the primary cell wall of Norway spruce (Picea abies (L.) Karst.) using dynamic FT-IR spectroscopy

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Abstract

Dynamic Fourier Transform Infra-Red (FT-IR) spectroscopy was used to examine the interactions among cellulose, xyloglucan, pectin, protein and lignin in the outer fibre wall layers of spruce wood tracheids. Knowledge regarding these interactions is fundamental for understanding the fibre separation in a mechanical pulping process. Sheets made from an enriched primary cell wall material were used for studying the viscoelastic response of the polymers. The results indicated that strong interactions exist among lignin, protein, pectin, xyloglucan and cellulose in the primary cell wall. This signified a closely linked network structure of the components on the fibre surface. This ultrastructural arrangement in the primary cell wall and the relatively high content of lignin, pectin and protein in it, means that the primary cell wall is more submissive to selective chemical attacks, when compared to the secondary cell wall. A low ratio of cellulose Iα to cellulose Iβ in the primary cell wall was also found.

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Abbreviations

FT-IR:

Fourier Transform Infra-Red

TMP:

Thermomechanical pulp

CTMP:

Chemithermomechanical pulp

P:

Primary cell wall

S1 :

Outer secondary cell wall

CSF:

Canadian standard freeness

BDDJ:

Britt dynamic drainage jar

S2 :

Middle secondary cell wall

RH:

Relative humidity

DMA:

Dynamic mechanical analysis

CP/MAS 13C-NMR:

Cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance

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Acknowledgements

This work was carried out within the framework of the Wood Ultrastructure Research Center (WURC) at Uppsala, Sweden, financed by VINNOVA, the Nordic pulp and paper industry and SLU. The authors wish to express their gratitude to Dr. Margaretha Åkerholm for precious advice concerning the dynamic FTIR spectroscopy. We are indebted to Ms. Joanna Hornatowska for kindly carrying out the light microscopy images. Dr. Tomas Larsson is especially acknowledged for performing the NMR measurements and valuable discussions.

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  1. STFI-Packforsk, Swedish Pulp and Paper Research Institute, Box 5604, Stockholm, 114 86, Sweden

    Jasna S. Stevanic & Lennart Salmén

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  1. Jasna S. Stevanic
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  2. Lennart Salmén
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Correspondence to Jasna S. Stevanic.

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Stevanic, J.S., Salmén, L. Characterizing wood polymers in the primary cell wall of Norway spruce (Picea abies (L.) Karst.) using dynamic FT-IR spectroscopy. Cellulose 15, 285–295 (2008). https://doi.org/10.1007/s10570-007-9169-1

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