Abstract
In this study we analyzed gene expression in 3T3-F442A pre-adipocyte cells that differentiate in the presence of micro-molar arsenate concentration. Two concentrations of arsenite (As2O3, 0.25 μmol/L and 0.5 μmol/L) were applied for three days with and without insulin (170 nmol/L) and gene expressions were evaluated by quantitative RT-PCR. The genes included genes of oxidative-stress responses: heme-oxygenase-1 (HO1) and the hypoxia inducible factor 1a (HIF1α), genes of cell-cycle: c-jun and Kruppel like factor 5 (KLF5), and genes that play important roles in adipose determination: a peroxisome proliferator-activated receptor (PPARγ) and a CCAAT/ enhancer binding protein (C/EBPα). Arsenite induced the expression of HO1, HIF1α, KLF5, PPARγ and C/EBPα. These results suggest that under condition of oxidative stress arsenite induces genes that are required for adipose differentiation.
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Salazard, B., Bellon, L., Jean, S. et al. Low-level arsenite activates the transcription of genes involved in adipose differentiation. Cell Biol Toxicol 20, 375–385 (2004). https://doi.org/10.1007/s10565-004-1471-1
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DOI: https://doi.org/10.1007/s10565-004-1471-1