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Epoxidation of Fatty Acids by Pluronic-Conjugated Lipase in Organic Media

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Abstract

In the present study, Candida antarctica lipase B (CALB) was conjugated with Pluronic polymers, a family of amphiphilic triblock copolymers made of a central hydrophobic polypropylene oxide block connected to two hydrophilic poly(ethylene oxide) side blocks, and tested for the chemo-enzymatic epoxidation of fatty acids conducted in organic media. The effects of operation parameters including the type of Pluronic polymers and solvents on the epoxidation of different plant oil substrates were examined, in which Pluronic F127 and toluene appeared the best polymer and solvent, respectively. At optimized conditions, an yield of 97, 75, 67 % for epoxidized oleic acid, linoleic acid, linolenic acid, respectively, was obtained using Pluronic F127 conjugated CALB. This study suggested that CALB-Pluronic conjugate is an appealing enzyme catalyst for the epoxidation in organic media.

Graphical Abstract

An enzymatic approach to epoxidation of fatty acids was investigated using a lipase-Pluronic conjugate as the catalyst.

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Acknowledgments

This work was supported by Tsinghua University Foundation 20141081143.

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Authors and Affiliations

  1. Key Lab of Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

    Oda Yuki, Yifei Zhang, Jun Ge & Zheng Liu

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  1. Oda Yuki
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  2. Yifei Zhang
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  3. Jun Ge
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  4. Zheng Liu
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Correspondence to Zheng Liu.

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Yuki, O., Zhang, Y., Ge, J. et al. Epoxidation of Fatty Acids by Pluronic-Conjugated Lipase in Organic Media. Catal Lett 146, 1073–1078 (2016). https://doi.org/10.1007/s10562-016-1726-5

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  • DOI: https://doi.org/10.1007/s10562-016-1726-5

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