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Antioxidant effects of chrysin-loaded electrospun nanofibrous mats on proliferation and stemness preservation of human adipose-derived stem cells

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Abstract

An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. Chrysin (Chr) is a naturally occurring flavone with a wide spectrum of biological functions including anti-inflammatory and anti-oxidant properties. The present study describes the influence of Chr-loaded nanofibrous mats on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). For this purpose, Chr-loaded poly (ε-caprolactone)/poly (ethylene glycol) (PCL/PEG) nanofibrous mats were produced via electrospinning process and the successful fabrication of these bioactive mats was confirmed by field emission scanning electron microscopy (FE-SEM) and fourier transform infrared spectroscopy. ADSCs were seeded on the nanofibers and their morphology, viability, and stemness expression were analyzed using FE-SEM, MTT, and qPCR assays after 2 weeks of incubation, respectively. The results display that ADSCs exhibit better adhesion and significantly increased viability on the Chr-loaded PCL/PEG nanofibrous mats in relative to the PCL/PEG nanofibers and tissue culture polystyrene. The greater viability of ADSCs on Chr based nanofibers was further confirmed by higher expression levels of stemness markers Sox-2, Nanog, Oct-4, and Rex-1. These findings demonstrate that Chr-loaded PCL/PEG electrospun nanofibrous mats can be applied to improve cell adhesion and proliferation while concurrently preserving the stemness of ADSCs, thus representing a hopeful potential for application in stem cell therapy strategies.

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Acknowledgements

Authors would like to thank Stem Cell Research Center, Tabriz University for supporting this project. Also, Authors gratefully acknowledge National Institute for Medical Research Development, Tehran, Iran for providing the necessary infrastructure for successful accomplishment of this research.

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Authors and Affiliations

  1. Department of Clinical Biochemistry and Laboratory Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

    Yaghoub Deldar, Faraz Zarghami & Nosratollah Zarghami

  2. National Institute for Medical Research Development, Tehran, Iran

    Yaghoub Deldar, Faraz Zarghami, Younes Pilehvar-Soltanahmadi & Nosratollah Zarghami

  3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Yaghoub Deldar, Younes Pilehvar-Soltanahmadi, Mehdi Dadashpour & Nosratollah Zarghami

  4. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

    Younes Pilehvar-Soltanahmadi, Mehdi Dadashpour & Nosratollah Zarghami

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  1. Yaghoub Deldar
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  2. Faraz Zarghami
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  3. Younes Pilehvar-Soltanahmadi
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  4. Mehdi Dadashpour
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  5. Nosratollah Zarghami
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Correspondence to Nosratollah Zarghami.

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Yaghoub Deldar and Faraz Zarghami are co-first author.

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Deldar, Y., Zarghami, F., Pilehvar-Soltanahmadi, Y. et al. Antioxidant effects of chrysin-loaded electrospun nanofibrous mats on proliferation and stemness preservation of human adipose-derived stem cells. Cell Tissue Bank 18, 475–487 (2017). https://doi.org/10.1007/s10561-017-9654-1

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  • DOI: https://doi.org/10.1007/s10561-017-9654-1

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