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Viability of pulp stromal cells in cryopreserved deciduous teeth

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Abstract

The cryopreservation of exfoliated deciduous teeth and harvesting of stem cells from them as required would reduce the costs and efforts associated with banking stem cells from primary teeth. The aim of this study was determine whether the viability of pulp stromal cells from deciduous teeth was influenced by the cryopreservation process itself or the period of cryopreservation. In total, 126 deciduous teeth were divided into three groups: (1) fresh, (2) cryopreserved for <3 months (cryo<3), and (3) cryopreserved for 3–9 months (cryo3–9). The viability of the pulp tissues was compared among the three groups by evaluating the outgrowth from pulp tissues and cell activity within those pulp tissues. In addition, the terminal deoxynucleotidyl transferase-mediated dUTP–biotin nick end labeling (TUNEL) assay was performed to compare cell apoptosis within fresh pulp tissue and pulp tissue that had been cryopreserved for 4 months. The outgrowth from and cell activity within the pulp tissues did not differ significantly between the fresh and cryo<3 pulp tissues. However, these parameters were significantly reduced in the cryo3–9 pulp tissue. In TUNEL assay, 4-month cryopreserved pulp tissues has more apoptotic cells than fresh group. In conclusion, it is possible to acquire pulp stromal cells from cryopreserved deciduous teeth. However, as the period of cryopreservation becomes longer, it is difficult to get pulp cells due to reduced cell viability.

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Acknowledgments

This study was supported by a faculty research grant of Yonsei University College of Dentistry for 2011 (No. 6-2011-0050). There were no competing financial interests.

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Authors and Affiliations

  1. Department of Pediatric Dentistry, College of Dentistry, Yonsei University, 250 Seongsanno, Seodaemun-gu, Seoul, 120-752, Korea

    Eun Hye Ji, Je Seon Song, Seong-Oh Kim, Byung-Jai Choi & Jae Ho Lee

  2. Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, Korea

    Eun Hye Ji, Je Seon Song, Seong-Oh Kim, Mijeong Jeon, Byung-Jai Choi & Jae Ho Lee

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  1. Eun Hye Ji
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  2. Je Seon Song
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  3. Seong-Oh Kim
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Correspondence to Jae Ho Lee.

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Eun Hye Ji and Je Seon Song equally contributed to this work.

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Ji, E.H., Song, J.S., Kim, SO. et al. Viability of pulp stromal cells in cryopreserved deciduous teeth. Cell Tissue Bank 15, 67–74 (2014). https://doi.org/10.1007/s10561-013-9375-z

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