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In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture

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Abstract

There have been many attempts to acquire and culture human keratinocytes for clinical purposes including from keratotome slices in media with fetal calf serum (FCS) or pituitary extract (PE), from skin specimens in media with feeder layers, from suction blister epidermal roofs’ in serum-free culture and from human umbilical cord blood (hUCB) mesenchymal stem cells (MSCs) in media with skin feeder layers. Conversely this study was designed to investigate whether keratinocytes could be obtained directly from hUCB MSCs in vitro. It is widely established that mesenchymal stem cells from human umbilical cord blood have multipotent capacity and the ability to differentiate into disparate cell lineages hUCB MSCs were directly induced to differentiate into keratinocytes by using a specific medium composed of primary culture medium (PCM) and serum free medium (SFM) in a ratio 1:9 for a period of 7 days and tested by immunostain p63 and K1-K10. Cells thus cultured were positive in both tests, confirming the possibility to directly obtain keratinocytes from MSCs hUCB in vitro.

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Authors and Affiliations

  1. Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam

    Tran Cong Toai, Huynh Duy Thao, Nguyen Phuong Thao, Tran Thi Thanh Thuy & Huynh Minh Tuan

  2. University of Western Australia School of Anatomy and Human Biology, Crawley, WA, Australia

    Ciro Gargiulo & Luis Filgueira

  3. Department of Orthopaedics and Sport Medicine, University of Washington School of Medicine, Seattle, WA, USA

    D. Micheal Strong

  4. Department of Pathology, Children No 1 Hospital in HCMC, Ho Chi Minh City, Vietnam

    Nguyen Thanh Tung

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  1. Tran Cong Toai
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  2. Huynh Duy Thao
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  3. Ciro Gargiulo
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  4. Nguyen Phuong Thao
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  5. Tran Thi Thanh Thuy
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  6. Huynh Minh Tuan
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  7. Nguyen Thanh Tung
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  8. Luis Filgueira
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  9. D. Micheal Strong
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Correspondence to Tran Cong Toai.

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Toai, T.C., Thao, H.D., Gargiulo, C. et al. In vitro culture of Keratinocytes from human umbilical cord blood mesenchymal stem cells: the Saigonese culture. Cell Tissue Bank 12, 125–133 (2011). https://doi.org/10.1007/s10561-010-9174-8

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  • DOI: https://doi.org/10.1007/s10561-010-9174-8

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