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In vitro culture and differentiation of osteoblasts from human umbilical cord blood

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Abstract

It is well accepted that human umbilical cord blood (UCB) is a source of mesenchymal stem cells (MSCs) which are able to differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. The aim of this study was to isolate MSCs from human UCB to determine their osteogenic potential by using different kinds of osteogenic medium. Eventually, only those MSCs cultured in osteogenic media enriched with vitamin D2 and FGF9, were positive for osteocalcin by RT-PCR. All these cells were positive for alizarin red, alkaline phosphatase and Von Kossa. The results obtained from RT-PCR have confirmed that osteogenesis is complete by expression of the osteocalcin marker. In conclusion, vitamin D2, at least in vitro, may replace vitamin D3 as an osteogenic stimulator factor for MSC differentiation.

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Authors and Affiliations

  1. Department of Histo-pathology, Embryology, Genetics and Biotechnology for Tissue Transplants, Pham Ngoc Thach Medical University, Ho Chi Minh City, Vietnam

    Tran Cong Toai, Huynh Duy Thao, Nguyen Phuong Thao & Ciro Gargiulo

  2. University of Natural Science, Ho Chi Minh City, Vietnam

    Phan Kim Ngoc

  3. The Central Lab of the University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam

    Pham Hung Van

  4. Department of Orthopaedics and Sports Medicine, University of Washington School of Medicine, Seattle, WA, 98195, USA

    D. Michael Strong

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  1. Tran Cong Toai
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  2. Huynh Duy Thao
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  3. Nguyen Phuong Thao
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  4. Ciro Gargiulo
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  5. Phan Kim Ngoc
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  6. Pham Hung Van
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  7. D. Michael Strong
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Correspondence to D. Michael Strong.

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Toai, T.C., Thao, H.D., Thao, N.P. et al. In vitro culture and differentiation of osteoblasts from human umbilical cord blood. Cell Tissue Bank 11, 269–280 (2010). https://doi.org/10.1007/s10561-009-9141-4

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  • DOI: https://doi.org/10.1007/s10561-009-9141-4

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