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Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis

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Abstract

The viscoelastic and inertial properties of the arterial wall are responsible for the arterial functional role in the cardiovascular system. Cryopreservation is widely used to preserve blood vessels for vascular reconstruction but it is controversially suspected to affect the dynamic behaviour of these allografts. The aim of this work was to assess the cryopreservation's effects on human arteries mechanical properties. Common carotid artery (CCA) segments harvested from donors were divided into two groups: Fresh (n = 18), tested for 24–48 h after harvesting, and Cryopreserved (n = 18) for an average time of 30 days in gas-nitrogen phase, and finally defrosted. Each segment was tested in a circulation mock, and its pressure and diameter were registered at similar pump frequency, pulse and mean pressure levels, including those of normotensive and hipertensive conditions. A compliance transfer function (diameter/pressure) derived from a mathematical adaptive modelling was designed for the on line assessment of the arterial wall dynamics and its frequency response. Assessment of arterial wall dynamics was made by measuring its viscous (η), inertial (M) and elastic (E) properties, and creep and stress relaxation time constant (τC and τSR, respectively). The frequency response characterization allowed to evaluate the arterial wall filter or buffer function. Results showed that non-significant differences exist between wall dynamics and buffer function of fresh and cryopreserved segments of human CCA. In conclusion, our cryopreservation method maintains arterial wall functional properties, close to their fresh values.

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Abbreviations

BF:

arterial buffering function

CF:

arterial conduit function

ARX:

autoregressive with exogenous input model

CCA:

common carotid arteries

τC :

creep time constant

f C :

cut-off frequency

E :

elastic index

ECMM:

esternal cleido mastoid muscle

M :

inertial index

τSR :

stress relaxation time constant

η :

viscous index

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Authors and Affiliations

  1. Departamento de Fisiología, Facultad de Medicina, Universidad de la República, General Flores 2125, PC:11800, Montevideo, Uruguay

    Daniel Bia, Ricardo Armentano & Yanina Zócalo

  2. Facultad de Ingeniería y Ciencias Exactas y Naturales, Universidad Favaloro, Solís, 453, C1078AAI, Buenos Aires, Argentina

    Franco Pessana, Ricardo Armentano & Sebastián Graf

  3. Banco Nacional de Órganos y Tejidos, Ministerio de Salud Pública, Facultad de Medicina, Montevideo, Uruguay

    Héctor Pérez, Maria Saldías, Natalia Perez, Oscar Alvarez, Walter Silva, Daniel Machin, Patricia Sueta, Silvia Ferrin, Maria Acosta & Inés Alvarez

  4. Facultad de Medicina, Universidad de la Républica, Avenida Italia 2870, Hospital de Clínicas, Montevideo, Uruguay

    Héctor Pérez, Maria Saldías, Natalia Perez, Oscar Alvarez, Walter Silva, Daniel Machin, Patricia Sueta, Silvia Ferrin, Maria Acosta & Inés Alvarez

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  1. Daniel Bia
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Correspondence to Daniel Bia.

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Bia, D., Pessana, F., Armentano, R. et al. Cryopreservation procedure does not modify human carotid homografts mechanical properties: an isobaric and dynamic analysis. Cell Tissue Banking 7, 183–194 (2006). https://doi.org/10.1007/s10561-005-0655-0

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