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The protective effect of intermittent calorie restriction on mammary tumorigenesis is not compromised by consumption of a high fat diet during refeeding

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Abstract

Previously we reported that intermittent calorie restriction (ICR) provided greater prevention of mammary tumors (MTs) than chronic calorie restriction (CCR). Here the impact of increased fat intake during refeeding in an ICR protocol was evaluated. MMTV-TGF-α female mice were assigned to one of three groups: ad libitum (AL) fed (n = 45) with free access to a moderately high fat diet (22 % fat calories); ICR (n = 45) 50 % calorie restricted for 3-week intervals followed by 3 weeks of 100 % of AL intake; and CCR (n = 45) fed 75 % of AL mice, matching each 6-week cycle of ICR mice. ICR mice were further designated as ICR-Restricted or ICR-Refed for data obtained during these intervals. All mice consumed the same absolute amount of dietary fat. Mice were followed to assess MT incidence, body weight and serum IGF-1, IGFBP3, leptin and adiponectin levels until 79 (end of final 3-week restriction) or 82 (end of final 3-weeks refeeding) weeks of age. Age of MT detection was significantly extended for CCR (74 weeks) and ICR (82 weeks) mice, compared to 57.5 weeks for AL mice. MT incidence for AL, ICR and CCR mice was 66.7, 4.4, and 52.3 %, respectively. Mammary and fat pad weights were reduced significantly following 50 % calorie restriction in ICR-Restricted mice compared to AL, CCR and ICR-Refed mice. IGF-1 and leptin levels also tended to be reduced in ICR-Restricted mice over the course of the study while adiponectin was not compared to AL, CCR, and ICR-Refed mice. The adiponectin:leptin ratio was consistently higher following 50 % restriction in ICR-Restricted mice. There was no relationship of IGF-1, leptin, or adiponectin with the presence of MTs in any groups. Thus the manner in which calories are restricted impacts the protective effect of calorie restriction independently of high fat intake.

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Abbreviations

ICR:

Intermittent calorie restriction

CCR:

Chronic caloric restriction

AL:

ad libitum

IGF-1:

Insulin-like growth factor-1

IGFBP-3:

Insulin-like growth factor binding protein-3

MMTV-TGF-α:

Mouse mammary tumor virus transforming growth factor-α

MT:

Mammary tumor

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Acknowledgments

This research was supported by NIH-CA 101858 and the Hormel Foundation.

Conflict of interest

We declare that we do not have a financial relationship with the organizations that sponsored the research.

Ethical standards

All procedures with mice were performed under the guidelines and with approval of the University of Minnesota Institutional Animal Care and Use Committee. The University of Minnesota is an AAALAC accredited institution.

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Authors and Affiliations

  1. The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN, 55912, USA

    Olga P. Rogozina, Katai J. Nkhata, Emily J. Nagle & Margot P. Cleary

  2. Mayo Clinic, Rochester, MN, 55905, USA

    Joseph P. Grande

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  1. Olga P. Rogozina
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  2. Katai J. Nkhata
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  3. Emily J. Nagle
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  4. Joseph P. Grande
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  5. Margot P. Cleary
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Correspondence to Margot P. Cleary.

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Rogozina, O.P., Nkhata, K.J., Nagle, E.J. et al. The protective effect of intermittent calorie restriction on mammary tumorigenesis is not compromised by consumption of a high fat diet during refeeding. Breast Cancer Res Treat 138, 395–406 (2013). https://doi.org/10.1007/s10549-013-2464-7

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