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Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models

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Abstract

Identifying the gene expression alterations that occur in both the tumor and stroma is essential to understanding tumor biology. We have developed a dual-species microarray analysis method that allows the dissection of both tumor and stromal gene expression profiles from xenograft models, based on limited interspecies cross-hybridization on Illumina gene expression beadchips. This methodology allows for simultaneous genome-wide analysis of gene expression profiles of both tumor cells and the associated stromal tissue.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Medicine, University of Miami Miller School of Medicine, 1501 NW 10th Ave (BRB 701), Miami, FL, 33136, USA

    Elizabeth Iorns, Jennifer Clarke, Toby Ward, Sonja Dean & Marc Lippman

  2. Department of Epidemiology and Public Health, University of Miami Miller School of Medicine, Miami, FL, 33136, USA

    Jennifer Clarke

Authors
  1. Elizabeth Iorns
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  2. Jennifer Clarke
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  3. Toby Ward
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  4. Sonja Dean
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  5. Marc Lippman
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Corresponding author

Correspondence to Elizabeth Iorns.

Additional information

Significance

The novel methodology described provides a simple method for simultaneously analyzing gene expression profiles from both tumor cells and the associated stromal tissue. This will significantly enhance the ability of researchers to identify important tumor–host interactions that regulate tumor development and progression.

Elizabeth Iorns and Jennifer Clarke contributed equally to this work.

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Iorns, E., Clarke, J., Ward, T. et al. Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models. Breast Cancer Res Treat 131, 321–324 (2012). https://doi.org/10.1007/s10549-011-1784-8

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  • DOI: https://doi.org/10.1007/s10549-011-1784-8

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