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RETRACTED ARTICLE: Glypican-3 reexpression regulates apoptosis in murine adenocarcinoma mammary cells modulating PI3K/Akt and p38MAPK signaling pathways

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This article was retracted on 04 September 2023

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Abstract

Glypican-3 (GPC3) is a proteoglycan involved in proliferation and cell survival. Several reports demonstrated that GPC3 is downregulated in some tumors, such as breast cancer. Previously, we determined that GPC3 reexpression in the murine mammary adenocarcinoma LM3 cells induced an impairment of their invasive and metastatic capacities, associated with a decrease of their motility and an increase of their cell death. We demonstrated that GPC3 inhibits canonical Wnt signaling, as well as it activates non canonical pathway. Now, we identified signaling pathways responsible for the pro-apoptotic role of GPC3 in LM3 cells. We found for the first time that GPC3 inhibits the PI3K/Akt anti-apoptotic pathway while it stimulates the p38MAPK stress-activated one. We report a concomitant modulation of CDK inhibitors as well as of pro- and anti-apoptotic molecules. Our results provide new clues regarding the mechanism involved in the modulation induced by GPC3 of mammary tumor cell growth and survival.

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Acknowledgments

We would like to give our thanks to Guillermo Peluffo for technical assistance and to Dr. Mariana Salatino for her invaluable assistance in responding the reviewer requests. The work was supported by grants from FONCyT (PICT 14088, Préstamo BID 1728/OC-AR; PICT 00220, Préstamo BID 1728/OC-AR) and from the University of Buenos Aires (UBACyT M068).

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Authors and Affiliations

  1. Cell Biology Department, Research Area, Institute of Oncology “Angel H. Roffo”, University of Buenos Aires, Av. San Martín 5481, C1417DTB, Buenos Aires, Argentina

    C. Buchanan, I. Stigliano, L. Puricelli, E. Bal de Kier Joffé & M. G. Peters

  2. School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, SP, 05508-900, Brazil

    H. M. Garay-Malpartida

  3. Chemistry Institute, University of São Paulo, São Paulo, SP, 05508-900, Brazil

    L. Rodrigues Gomes & M. C. Sogayar

Authors
  1. C. Buchanan
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  2. I. Stigliano
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  3. H. M. Garay-Malpartida
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  4. L. Rodrigues Gomes
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  5. L. Puricelli
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  6. M. C. Sogayar
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  7. E. Bal de Kier Joffé
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  8. M. G. Peters
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Corresponding author

Correspondence to M. G. Peters.

Additional information

C. Buchanan, L. Puricelli, E. Bal de Kier Joffé, and M. G. Peters are Members of the National Council of Scientific and Technical Research (CONICET).

C. Buchanan and I. Stigliano contributed equally to this paper.

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Buchanan, C., Stigliano, I., Garay-Malpartida, H.M. et al. RETRACTED ARTICLE: Glypican-3 reexpression regulates apoptosis in murine adenocarcinoma mammary cells modulating PI3K/Akt and p38MAPK signaling pathways. Breast Cancer Res Treat 119, 559–574 (2010). https://doi.org/10.1007/s10549-009-0362-9

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  • DOI: https://doi.org/10.1007/s10549-009-0362-9

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