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Concentration-dependent effects of genistein on global gene expression in MCF-7 breast cancer cells: an oligo microarray study

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Abstract

Breast cancer is the most commonly diagnosed cancer among US women; there is therefore great interest in developing preventive and treatment strategies for this disease. Because breast cancer incidence is much lower in countries where women consume high levels of soy, bioactive compounds in this food source have been studied for their effects on breast cancer. Genistein, found at high levels in soybeans and soy foods, is a controversial candidate breast cancer preventive phytochemical whose effects on breast cells are complex. To understand more clearly the molecular mechanisms underlying the effects of genistein on breast cancer cells, we used a DNA oligo microarray approach to examine the global gene expression patterns in MCF-7 breast cancer cells at both physiologic (1 or 5 μM) and pharmacologic (25 μM) genistein concentrations. Microarray analyses were performed on MCF-7 cells after 48 h of either vehicle or 1, 5, or 25 μM genistein treatment. We found that genistein altered the expression of genes belonging to a wide range of pathways, including estrogen- and p53-mediated pathways. At 1 and 5 μM, genistein elicited an expression pattern suggestive of increased mitogenic activity, confirming the proliferative response to genistein observed in cultured MCF-7 cells, while at 25 μM genistein effected a pattern that likely contributes to increased apoptosis, decreased proliferation and decreased total cell number, also consistent with cell culture results. These findings provide evidence for a molecular signature of genistein’s effects in MCF-7 cells and lay the foundation for elucidating the mechanisms of genistein’s biological impact in breast cancer cells.

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Acknowledgments

Partial funding for this project was provided by a grant to SDH from the Breast Cancer Research Foundation. JAL would like to acknowledge support from the National Cancer Institutes Cancer Prevention Fellowship Program while carrying out this project.

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Authors and Affiliations

  1. Division of Cancer Prevention, National Cancer Institute, NIH, Bethesda, MD, USA

    Jackie A. Lavigne

  2. Beltsville Human Nutrition Research Center, ARS, USDA, National Food Research Institute, Tsukuba, Ibaraki, Japan and Phytonutrients Laboratory, Beltsville, MD, USA

    Yoko Takahashi

  3. Office of the Director, National Cancer Institute, NIH, Bethesda, MD, USA

    Gadisetti V. R. Chandramouli

  4. Center for Bioinformatics/SAIC, NCI, NIH, Bethesda, MD, USA

    Huaitian Liu

  5. Division of Nutritional Sciences, University of Texas at Austin, Austin, TX, USA

    Susan N. Perkins

  6. Division of Nutritional Sciences, University of Texas at Austin, Austin, TX, USA

    Stephen D. Hursting

  7. Phytonutrients Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Building 307C, Room 132, Beltsville, MD, 20705, USA

    Thomas T. Y. Wang

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  1. Jackie A. Lavigne
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  2. Yoko Takahashi
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  7. Thomas T. Y. Wang
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Correspondence to Thomas T. Y. Wang.

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Lavigne, J.A., Takahashi, Y., Chandramouli, G.V.R. et al. Concentration-dependent effects of genistein on global gene expression in MCF-7 breast cancer cells: an oligo microarray study. Breast Cancer Res Treat 110, 85–98 (2008). https://doi.org/10.1007/s10549-007-9705-6

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