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Celecoxib Inhibits Urokinase-Type Plasminogen Activator (uPA) Production in MDA-MB-231 Breast Cancer Cells

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Summary

Elevated urokinase-type plasminogen activator (uPA) expression in breast tumors predicts poor survival. We found celecoxib (25 μM) significantly reduced uPA protein and mRNA in MDA-MB-231 breast cancer cells following 72 h of treatment. Celecoxib also inhibited cell viability (12.5 and 25 μM) and induced G2M arrest (25 μM). Therefore, celecoxib therapy for uPA positive breast cancer should be explored.

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Abbreviations

COX-2:

cyclo-oxygenase II

ELISA:

enzyme linked immunosorbent assay

Erk 1/2:

extra-cellular regulated kinase 1 and 2

IGF-I:

insulin-like growth factor I

IGF-IR:

IGF-I receptor

MAPK I:

mitogen-activated protein kinase I

MTS:

3-(4,5 dimethylthiazol-2-yl)-5-(3 carboxymethoxyphenyl)-2-(4 sulfophenyl)-2H-tetrazolium

NSAID:

nonsteroidal anti-inflammatory drug, PI3K phosphatidyl-inositol 3 kinase

qRT-PCR:

quantitative real time polymerase chain reaction

TBP:

TATA box binding protein

TGF-beta:

transforming growth factor-beta

uPA:

urokinase-type plasminogen activator

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Authors and Affiliations

  1. Department of Pediatrics, British Columbia Institute for Children’s and Women’s Health, Vancouver, BC, Canada

    Heather N. Andrews, Golerah Habibi, Jill E. Kucab & Sandra E. Dunn

  2. Department of Pediatrics, British Columbia Institute for Children’s and Women’s Health, 950 West 28th Ave, Vancouver, BC, V5Z 4H4, Canada

    Sandra E. Dunn

Authors
  1. Heather N. Andrews
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  2. Golerah Habibi
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  3. Jill E. Kucab
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  4. Sandra E. Dunn
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Correspondence to Sandra E. Dunn.

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Andrews, H.N., Habibi, G., Kucab, J.E. et al. Celecoxib Inhibits Urokinase-Type Plasminogen Activator (uPA) Production in MDA-MB-231 Breast Cancer Cells. Breast Cancer Res Treat 94, 47–52 (2005). https://doi.org/10.1007/s10549-005-6941-5

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  • DOI: https://doi.org/10.1007/s10549-005-6941-5

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