Abstract
An integrated microsystem device with matched interdigitated microelectrode chip was fabricated for enrichment and detection of Escherichia coli O157:H7. The microsystem has integrated with positive dielectrophoresis (pDEP) enrichment and in situ impedance detection, whose total volume is only 3.0 × 10−3 m3, and could provide impedance testing voltages of 0 ~ 10 V, detection frequencies of 1 KHz ~ 1 MHz, DEP excitation signals with amplitude of 0 ~ 10 Vpp and frequencies of 1KHz ~ 1 MHz, which fully meets the demands of pDEP enrichment and impedance detection for bacteria. The microfluidic chip with interdigitated microelectrodes was manufactured by microfabrication methods. The interdigital microelectrode array has sufficient contact area with a bacterial suspension to improve enrichment efficiency and detection sensitivity. Bacteria in the interdigital microelectrode area of the microfluidic chip were firstly captured and enriched by pDEP. Then, in situ impedance detection of the enriched bacteria was realized by switching test conditions. Using the self-assembly microsystem, a novel quantitative detection method was established and demonstrated to detect Escherichia coli O157:H7. Experimental results showed that the detection limits of Escherichia coli O157:H7 was 5 × 104 cfu mL−1, and testing time was only 6 min under the optimized detection voltage of 100 mV and frequency of 500 KHz. The method was successfully used to detect Escherichia coli O157:H7 in synthetic chicken synthetic samples.
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References
M.J. Allen, S.C. Edberg, D.J. Reasoner, Int. J. Food Microbiol. 92, 265–274 (2004)
J.Z. AnHong Zhou, Q.J. Xie, S.Z. Yao, Biomaterials 10 (2001)
C. Baek, H.Y. Kim, D. Na, J. Min, Sensors Actuators B Chem. 208, 238–244 (2015)
A. Bajwa, S.T. Tan, R. Mehta, B. Bahreyni, Sensors (Basel) 13, 8188–8198 (2013)
D.A. Boehm, P.A. Gottlieb, S.Z. Hua, Sensors Actuators B Chem. 126, 508–514 (2007)
W.H. Chang, C.H. Wang, C.L. Lin, J.J. Wu, M.S. Lee, G.B. Lee, Biosens. Bioelectron. 66, 148–154 (2015)
I.H. Cho, J. Irudayaraj, Int. J. Food Microbiol. 164, 70–75 (2013)
S.S. Donato, V. Chu, D.M. Prazeres, J.P. Conde, Electrophoresis 34, 575–582 (2013)
M. Dweik, R.C. Stringer, S.G. Dastider, Y. Wu, M. Almasri, S. Barizuddin, Talanta 94, 84–89 (2012)
C.F. Fronczek, D.J. You, J.Y. Yoon, Biosens. Bioelectron. 40, 342–349 (2013)
X. He, C. Hu, Q. Guo, K. Wang, Y. Li, J. Shangguan, Biosens. Bioelectron. 42, 460–466 (2013)
S. Jain, S. Chattopadhyay, R. Jackeray, C.K. Abid, G.S. Kohli, H. Singh, Biosens. Bioelectron. 31, 37–43 (2012)
J.H. Jung, G.Y. Kim, T.S. Seo, Lab Chip 11, 3465–3470 (2011)
S. Kim, G. Yu, T. Kim, K. Shin, J. Yoon, Electrochim. Acta 82, 126–131 (2012)
C.C. Liu, C.Y. Yeung, P.H. Chen, M.K. Yeh, S.Y. Hou, Food Chem. 141, 2526–2532 (2013)
H. Mollasalehi, R. Yazdanparast, Anal. Chim. Acta 770, 169–174 (2013)
N.J. Opet, R.E. Levin, J. Microbiol. Methods 94, 69–72 (2013)
Y. X. Renjie et al, T. Zhang, Y. Jiang, Analytical methods, doi:10.1039/C4AY02880E10.1039/c4ay02880e, 6, (2015).
Y. Song, H. Zhang, C.H. Chon, S. Chen, X. Pan, D. Li, Anal. Chim. Acta 681, 82–86 (2010)
J. Suehiro, M. Shutou, T. Hatano, M. Hara, Sensors Actuators B Chem. 96, 144–151 (2003)
J. Suehiro, A. Ohtsubo, T. Hatano, M. Hara, Sensors Actuators B Chem. 119, 319–326 (2006)
M.R. Tomkins, J. Chow, Y. Lai, A. Docoslis, Sensors Actuators B Chem. 176, 248–252 (2013)
M. Varshney and Y. Li, Biosensors & bioelectronics, 22, 2408–2414, (2007).
M. Varshney, Y. Li, Biosens. Bioelectron. 24, 2951–2960 (2009)
M. Varshney, Y. Li, B. Srinivasan, S. Tung, Sensors Actuators B Chem. 128, 99–107 (2007)
R.P. Vind, A.L. Inkar, R.K. Choudhury, B.K. Nayak, A. Saxena, R.G. Thomas, D.C. Biswas, B.V. John, Nuclear instruments and methods in physics research section a: Accelerators, spectrometers. Detect. Assoc Equip. 580, 1435–1440 (2007)
N. Wang, M. He, H.C. Shi, Anal. Chim. Acta 590, 224–231 (2007)
R. Wang, Y. Ni, Y. Xu, Y. Jiang, C. Dong, N. Chuan, Anal. Chim. Acta 853, 710–717 (2015)
L. Yang, Talanta 74, 1621–1629 (2008)
L. Yang, Talanta 80, 551–558 (2009)
L. Yang, Anal. Lett. 45, 187–201 (2012)
L. Yang, C. Ruan, Y. Li, Biosens. Bioelectron. 19, 495–502 (2003)
L. Yang, P.P. Banada, M.R. Chatni, K. Seop Lim, A.K. Bhunia, M. Ladisch, R. Bashir, Lab Chip 6, 896–905 (2006)
P. Zuo, X. Li, D.C. Dominguez, B.C. Ye, Lab Chip 13, 3921–3928 (2013)
Acknowledgements
The work was financially supported by National Natural Science Foundation of China (No. 21375156); National High Technology Research and Development Program of China (Ministry of Science and Technology 863 Plan) (No.2015AA021104); Frontier Research Key Projects of Chongqing Science and Technology Committee, [cstc2015jcyjBX0010]; Scientific and Technical Innovation Projects for People’s Livelihood of Chongqing Science and Technology Committee, [cstc2015shms,zx00014]; Benefit Projects for People’s Livelihood by Science and Technology, Chongqing Science and Technology Committee [cstc2015jcsf8001],2015.07-2017.07. Support the USDA-Purdue Center for Food Safety Engineering is appreciated.
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Wang, R., Xu, Y., Liu, H. et al. An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli . Biomed Microdevices 19, 34 (2017). https://doi.org/10.1007/s10544-017-0167-2
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DOI: https://doi.org/10.1007/s10544-017-0167-2