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Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates

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Abstract

This paper proposes a capture device to manipulate and transport a cellular aggregate in a micro-well. A cellular aggregate (a few hundreds μm in diameter) is currently manipulated by a pipette. The manual manipulation by a pipette has problems; low reliability, low throughput, and difficulty in confirmation of task completion. We took into account of compatibility with existing methods such as a micro-well-plate and designed for the capture device of a cellular aggregate in a micro-well. A newly developed capture device flows and carries a cellular aggregate from a bottom of a well to a trap of the capture device. We designed a curved surface at the bottom of the capture device to form a space to act as a channel between the inner wall of the micro-well. This paper presents concept, design, fabrication, and of the proposed cellular aggregate capture, followed by successful experimental results.

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Acknowledgments

This work was partially supported by Grant-in-Aid for Scientific Research (A) : JSPS KAKENHI Grant Number 24240075.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga, 525-8577, Japan

    Satoshi Konishi & Wataru Tonomura

  2. Graduate School of Science and Engineering, Ritsumeikan University, 1-1-1 Noji-higashi, Kusatsu, Shiga, 525-8577, Japan

    Yumi Teramachi & Shuhei Shimomura

  3. Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Shuhei Tajima & Yasuhiko Tabata

Authors
  1. Satoshi Konishi
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  2. Yumi Teramachi
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  3. Shuhei Shimomura
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  4. Wataru Tonomura
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  5. Shuhei Tajima
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  6. Yasuhiko Tabata
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Corresponding author

Correspondence to Satoshi Konishi.

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Konishi, S., Teramachi, Y., Shimomura, S. et al. Cellular aggregate capture by fluidic manipulation device highly compatible with micro-well-plates. Biomed Microdevices 17, 48 (2015). https://doi.org/10.1007/s10544-015-9953-x

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  • DOI: https://doi.org/10.1007/s10544-015-9953-x

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