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Optical biosensors with an integrated Mach-Zehnder Interferometer for detection of Listeria monocytogenes

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Abstract

In this work, we have demonstrated an efficient optical immunoassay technique for the detection of a food-borne pathogen, Listeria monocytogenes, using a Mach-Zehnder Interferometer (MZI) configuration. We have investigated ten different MZI configurations with angular and Sbend Y-junction geometries. An efficient Hydrofluoric acid (HF) based technique was used for rapid and specific binding of L. monocytogenes to the sensor arm of the MZI biosensor. The MZI biosensor was able to detect L. monocytogenes at concentrations of the order of 105 CFU/ml, which is lower than the infection dose for healthy human beings. SEM analysis and light intensity measurements showed the biosensor is highly selective to L. monocytogenes over other microbial species (such as Escherichia coli). Finally, a novel calibration scheme of the MZI biosensor was developed from experimental data that can be used for determining unknown concentrations of L. monocytogenes.

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Acknowledgments

The authors gratefully acknowledge the financial support from Natural Sciences and Engineering Council-Collaborative Research and Development (NSERC-CRD) program and AQL Management Consulting, Inc. We also gratefully acknowledge the useful discussions with Stefania Dante, PhD student at Research Center on Nanoscience and Nanotechnology(CIN2), Spanish National Research Council (CSIC), Barcelona, Spain.

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Authors and Affiliations

  1. Department of Mechanical Engineering, University of Alberta, Edmonton, T6G 2G8, Canada

    Dibyo Sarkar, Naga Siva Kumar Gunda & Sushanta K. Mitra

  2. AQL Management Consulting Inc., 4216 109A St NW, Edmonton, AB, T6J 2R8, Canada

    Iqbal Jamal

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  1. Dibyo Sarkar
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  2. Naga Siva Kumar Gunda
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  3. Iqbal Jamal
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  4. Sushanta K. Mitra
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Correspondence to Sushanta K. Mitra.

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Sarkar, D., Gunda, N.S.K., Jamal, I. et al. Optical biosensors with an integrated Mach-Zehnder Interferometer for detection of Listeria monocytogenes . Biomed Microdevices 16, 509–520 (2014). https://doi.org/10.1007/s10544-014-9853-5

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