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3D printing of biomimetic microstructures for cancer cell migration

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Abstract

To understand the physical behavior and migration of cancer cells, a 3D in vitro micro-chip in hydrogel was created using 3D projection printing. The micro-chip has a honeycomb branched structure, aiming to mimic 3D vascular morphology to test, monitor, and analyze differences in the behavior of cancer cells (i.e. HeLa) vs. non-cancerous cell lines (i.e. 10 T1/2). The 3D Projection Printing system can fabricate complex structures in seconds from user-created designs. The fabricated microstructures have three different channel widths of 25, 45, and 120 microns wide to reflect a range of blood vessel diameters. HeLa and 10 T1/2 cells seeded within the micro-chip were then analyzed for morphology and cell migration speed. 10 T1/2 cells exhibited greater changes in morphology due to channel size width than HeLa cells; however, channel width had a limited effect on 10 T1/2 cell migration while HeLa cancer cell migration increased as channel width decreased. This physiologically relevant 3D cancer tissue model has the potential to be a powerful tool for future drug discoveries and cancer migration studies

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Acknowledgments

The project described was supported by Award Number R01EB012597 from the National Institute of Biomedical Imaging And Bioengineering and a grant (CMMI-1130894) from the National Science Foundation. We thank the computer support from Intel’s High Education Program.

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Authors and Affiliations

  1. Department of NanoEngineering, University of California, MC-0448, SME Building 245B, 9500 Gilman Drive, La Jolla, San Diego, CA, 92093, USA

    Tina Qing Huang, Xin Qu & Shaochen Chen

  2. Department of Materials Science and Engineering, University of California, MC-0448, SME Building 245B, 9500 Gilman Drive, La Jolla, San Diego, CA, 92093, USA

    Justin Liu

Authors
  1. Tina Qing Huang
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  2. Xin Qu
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  3. Justin Liu
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  4. Shaochen Chen
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Correspondence to Shaochen Chen.

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Huang, T.Q., Qu, X., Liu, J. et al. 3D printing of biomimetic microstructures for cancer cell migration. Biomed Microdevices 16, 127–132 (2014). https://doi.org/10.1007/s10544-013-9812-6

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  • DOI: https://doi.org/10.1007/s10544-013-9812-6

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