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Olfactory epithelium biosensor: odor discrimination of receptor neurons from a bio-hybrid sensing system

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Abstract

Bio-hybrid systems provide an opportunity for integrating a living bio-active unit and a proper biosensing system, to employ the unique properties of the bio-active unit. The biological olfactory system can sense and identify thousands of trace odors. The purpose of this study is to combine olfactory epithelium with microelectrode array (MEA) to establish an olfactory epithelium-MEA hybrid system to record the odor-induced electrophysiological activities of the tissue. In our experiments, extracellular potential of olfactory receptor neurons in intact epithelium were measured in the presence of ethyl ether, acetic acid, butanedione, and acetone, respectively. After the odor-induced response signals were analyzed in the time and frequency domain, the temporal characteristics of response signals were extracted. We found that olfactory epithelium-MEA hybrid system can reflect the in vitro odor information of different signal characteristics and firing modes in vitro. The bio-hybrid sensing system can represent a useful instrument to sense and detect the odorant molecules with well recognizing patterns. With the development of sensor technology, bio-hybrid systems will represent emerging and promising platforms for wide applications, ranging from health care to environmental monitoring.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81027003, 81071226), the Research on Public Welfare Technology Application Projects of Zhejiang Province, China (No. 2011C23096), the Zhejiang Provincial Natural Science Foundation of China (No. Y2100684), and the Fundamental Research Funds for the Central Universities. The authors sincerely thank the anonymous reviewers and editors for their valuable comments that have led to the present improved version of the original manuscript.

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Authors and Affiliations

  1. Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, People’s Republic of China

    Qingjun Liu, Ning Hu, Fenni Zhang, Diming Zhang & Ping Wang

  2. Micro and Nanotechnology Laboratory, Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA

    Qingjun Liu & K Jimmy Hsia

  3. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, People’s Republic of China

    Ping Wang

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  1. Qingjun Liu
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  2. Ning Hu
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  3. Fenni Zhang
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  4. Diming Zhang
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  5. K Jimmy Hsia
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  6. Ping Wang
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Correspondence to Ping Wang.

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Liu, Q., Hu, N., Zhang, F. et al. Olfactory epithelium biosensor: odor discrimination of receptor neurons from a bio-hybrid sensing system. Biomed Microdevices 14, 1055–1061 (2012). https://doi.org/10.1007/s10544-012-9705-0

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  • DOI: https://doi.org/10.1007/s10544-012-9705-0

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